Anti-Human CD97/ADGRE5 (extracellular) Antibody | Alomone Labs AER-055 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CD97/ADGRE5 (extracellular) Antibody

catalog :

AER-055

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of HepG2 (lanes 1 and 5), Jurkat (lane 2 and 6), K562 (lanes 3 and 7) and THP-1 (lanes 4 and 8) cell lines lysates: - 1-4. Anti-Human CD97/ADGRE5 (extracellular) Antibody (#AER-055), (1:200). 5-8. Anti-Human CD97/ADGRE5 (extracellular) Antibody, preincubated with Human CD97/ADGRE5 (extracellular) Blocking Peptide (#BLP-ER055).

product information

CAT :

AER-055

SKU :

AER-055-CF_0.2 ml

Product Name :

Anti-Human CD97/ADGRE5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48960

Applications :

IFC WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-ER055

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KSDSDRGGHWATEG, corresponding to amino acid residues 499-512 of human CD97/ADGRE5

Product Page - Scientific background :

CD97, encoded by the adhesion G protein-coupled receptor 5 (ADGRE5) gene, is a member of the epidermal growth factor (EGF) seven transmembrane receptor family, belonging to the adhesion family of G protein-coupled receptors (GPCRs)1. CD97 is widely expressed on the surface of lymphoid cells, macrophages, smooth muscle cells and several types of tumor cells and acts through binding of decay accelerating factor (CD55) and dermatan sulfate2.In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation3. Antibodies against CD97 have been demonstrated to diminish various inflammatory disorders by depleting granulocytes and the interaction between CD97 and its ligand CD55 regulates T-cell activation and increases proliferation and cytokine production4.Changes in the expression of CD97 have been described in auto-inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Outside the immune system, CD97 is likely involved in cell-cell interactions and ectopic CD97 expression leading to enhanced cell-cell aggregation5.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation

Short Description :

A Rabbit Polyclonal Antibody to CD97/ADGRE5

Long Description :

Anti-Human CD97/ADGRE5 (extracellular) Antibody (#AER-055) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CD97 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

Negative Control :

BLP-ER055

Positive Control :

Synonyms :

Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADGRE5

AB Specifictiy :

The antibody will not recognize the protein from rat or mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage