Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody | Alomone Labs AER-051-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody

catalog :

AER-051-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human promyelocytic leukemia (HL-60) (lanes 1 and 5), human Burkitt's lymphoma (Raji) B (lanes 2 and 6), mouse macrophage J774 (lanes 3 and 7) and rat basophilic leukemia (RBL) (lanes 4 and 8) cell lysates: - 1-4. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200). 5-8. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).

product information

CAT :

AER-051-GP

SKU :

AER-051-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q14246

Applications :

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-ER051

Homology :

Mouse, Rat - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1

Product Page - Scientific background :

Human epidermal growth factor (EGF)-like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Short Description :

A Guinea Pig Polyclonal Antibody to EMR1

Long Description :

Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP, formerly AGP-214) raised in guinea pig, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGRE1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-ER051

Positive Control :

Synonyms :

MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADGRE1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage