Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody | Alomone Labs AER-051-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody

catalog :

AER-051-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of EMR1 in live intact mouse J774 macrophage cells: Cell surface detection of EMR1 in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody (#AER-051-F), (2.5 µg).

product information

CAT :

AER-051-F

SKU :

AER-051-F_50 mcl

Product Name :

Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q14246

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER051

Homology :

Mouse, Rat -11/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1

Product Page - Scientific background :

Human epidermal growth factor (EGF)-like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Short Description :

A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples. Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody (#AER-051-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADGRE1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AER-051), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage