Anti-GPER (GPR30) Antibody | Alomone Labs AER-050 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPER (GPR30) Antibody

catalog :

AER-050

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Sarchielli E, Guarnieri G, Idrizaj E, Squecco R, Mello T, Comeglio P, et al. The G protein-coupled oestrogen receptor, GPER1, mediates direct anti-inflammatory effects of oestrogens in human cholinergic neurones from the nucleus basalis of Meynert. J Neuroendocrinol. 2020;32:e12837 pubmed publisher
Ye Z, Cudmore R, Linden D. Estrogen-Dependent Functional Spine Dynamics in Neocortical Pyramidal Neurons of the Mouse. J Neurosci. 2019;39:4874-4888 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes: - 1-3. Anti-GPER (GPR30) Antibody (#AER-050), (1:200).4-6. Anti-GPER (GPR30) Antibody, preincubated with GPER/GPR30 Blocking Peptide (#BLP-ER050).

image 2 :

Expression of GPER in rat dentate gyrus - Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-GPER (GPR30) Antibody (#AER-050). A. GPER staining (green) appears in cells and fibers of the hilus (arrows) and granule layer (G) B. Parvalbumin (red) is detected in cells in the DG hilus (arrows). C. Nuclei staining using DAPI as the counterstain (blue). D. Merge image of panels A and B reveals colocalization in some cells (arrows).

product information

CAT :

AER-050

SKU :

AER-050-CF_0.2 ml

Product Name :

Anti-GPER (GPR30) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08878

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER050

Homology :

Mouse - 13/14; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER

Product Page - Scientific background :

The G protein-coupled estrogen receptor (GPER), formerly known as G protein receptor 30 (GPR30), was identified as a novel estrogen receptor that mediates a rapid, non-genomic response to estrogens1.GPER is a G protein-coupled seven-transmembrane receptor. It is believed that the N-terminus is located outside of the cell, and that aspartic acid residues in the terminal region might be modified by glycosylation if GPER is localized in the plasma membrane. It is speculated that the ligand associates with the N-terminal domain to activate the receptor. Trimeric G protein is presumed to bind to the 3rd loop of the intracellular domain based on the molecular structure. A PDZ domain appears to be in the C-terminal region of GPER, but its physiological role is unknown2.GPER is widely distributed in the human body including: brain, liver, heart, lung, pancreas, placenta, blood vessels, bone, lymphoid tissue, as well as endometrial, ovarian and breast cancer. The receptor has been localized both in the plasma membrane and in the endoplasmic reticulum3.It was found that GPER is rapidly induced in the cardiomyocyte ischemia model, and that its induction is associated with apoptotic cell death during ischemia and reperfusion4. In addition, the GPER mediates estrogenic signaling in breast cancer cells and cancer-associated fibroblasts (CAF) that contribute to cancer progression5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2

Short Description :

A Rabbit Polyclonal Antibody to G-Protein Coupled Estrogen Receptor 1

Long Description :

Anti-GPER (GPR30) Antibody (#AER-050) is a highly specific antibody directed against an epitope of rat G-protein coupled estrogen receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPER from rat, mouse and human samples.

Negative Control :

BLP-ER050

Positive Control :

Synonyms :

G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPER1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage