antibody

Anti-GPER1/GPR30 (extracellular)-FITC Antibody

AER-049-F

polyclonal

domestic rabbit

FITC

NA

human, mouse, rat

AER-049-F_15 mcl

Anti-GPER1/GPR30 (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

Q8BMP4

FC

Human Rat Mouse

Rabbit

BLP-ER049

Rat - identical; human - 13/14 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

3rd extracellular loop

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

CKQSFRHAYPLTGH, corresponding to amino acid residues 294 - 307 of mouse GPER1

GPER1/GPR30 is an estrogen receptor belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members, GPER1 has seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal domain. It is widely expressed in the central nervous system (CNS) and it binds 17β-estradiol (17β-E) with high affinity1.Estrogens have long been known as important regulators of the female reproductive functions, however, is now commonly accepted that estrogens and androgens have important functions in both female and male physiology and pathology.As opposed to the epigenetic regulatory role in estrogen signaling carried out by ER-α and ER-β estrogen receptors, signaling from GPER1 is responsible for the rapid non-genomic actions of estrogens that activate various protein-kinase cascades1. GPER1 signaling improves spatial memory, possibly via release of neurotransmitters. In addition, GPER1 activation contributes to behaviors that denote anxiety, and to social behaviors such as social memory and lordosis behavior in mice. In the male hippocampus, GPER1 activation leads to phosphorylation of the classical estrogen receptor ERα, suggesting that crosstalk with ERα is important in the manifestation of these behaviors, many of which are absent in ERα-null mice2. GPER1 activation also has several beneficial effects in the cardiovascular system including vasorelaxation, inhibition of smooth muscle cell proliferation, and protection of the myocardium against ischemia/reperfusion injury, and in the metabolic system including stimulation of insulin release and protection against pancreatic β-cell apoptosis. Thus, GPER1 is emerging as a candidate therapeutic target in both cardiovascular and metabolic diseases3.

FC

Alomone Labs

G Protein-Coupled Estrogen Receptor 1, G Protein-Coupled Receptor 30, Membrane Estrogen Receptor, Chemoattractant Receptor-Like 2, GPER

A Rabbit Polyclonal Antibody to GPER1 Conjugated to the Fluorescent Dye FITC

Anti-GPER1/GPR30 (extracellular) Antibody (#AER-049) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPER1 from rat, mouse, and human samples.  Anti-GPER1/GPR30 (extracellular)-FITC Antibody (#AER-049-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

RIC-001-F

NA

G Protein-Coupled Estrogen Receptor 1, G Protein-Coupled Receptor 30, Membrane Estrogen Receptor, Chemoattractant Receptor-Like 2, GPER

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

GPER1

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #AER-049), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information