Anti-Ephrin-B1 (extracellular) Antibody | Alomone Labs AER-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ephrin-B1 (extracellular) Antibody

catalog :

AER-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse enriched brain membranes (lanes 1 and 3) and human HT-29 colon adenocarcinoma cells lysate (lanes 2 and 4): - 1,2. Anti-Ephrin-B1 (extracellular) Antibody (#AER-041), (1:200).3,4. Anti-Ephrin-B1 (extracellular) Antibody, preincubated with Ephrin-B1 (extracellular) Blocking Peptide (#BLP-ER041).

image 2 :

Peptide (C)KHETVNQEEKSGPG corresponding to amino acid residues 208-221 of human Ephrin-B1 (AccessionP98172). Extracellular domain.

image 3 :

Peptide (C)EFRKKSGSAPPRK corresponding to amino acid residues 496-508 of rat Glucose Transporter 2 (AccessionP12336). Intracellular C-terminus.

product information

CAT :

AER-041

SKU :

AER-041-CF_0.2 ml

Product Name :

Anti-Ephrin-B1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P98172

Applications :

IFC LCI WB

Reactivity :

Human Rat

Host :

Rabbit

Blocking Peptide :

BLP-ER041

Homology :

Mouse - identical; rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KHETVNQEEKSGPG, corresponding to amino acid residues 208-221 of human Ephrin-B1

Product Page - Scientific background :

The erythropoietin-producing hepatocellular (EPH) receptors represent the largest known family of receptor tyrosine kinases and are activated by interactions with cell-surface ligands, termed EFNs. EPH receptors have been classified into two subfamilies, EPHA and EPHB, according to their preference for either glycosylphosphatidylinositol (GPI)-anchored EFN-A ligands or transmembrane EFN-B ligands. Thus far, 14 vertebrate receptors and eight ligands have been identified1. All the ligands share a conserved core sequence of approximately 125 amino acids, including 4 invariant cysteine residues, probably corresponding to a receptor binding domain. This is followed by a membrane anchorage domain, taking the form of a GPI anchor for five of the ligands (ephrin-A1 to -A5) or a transmembrane domain for three of them (ephrin-B1 to -B3) 2.Ephrin-B1 is signals forward or reverse signaling mechanisms. Ephrin-B1 PDZ-dependent reverse signaling controls axon guidance of the corpus callosum, whereas forward signaling is critical for normal craniofacial development2.Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they are involved in fundamental developmental processes of the nervous system, including axon guidance, axonfasciculation, neural crest cell migration, acquisition of brain subregional identity,and neuronal cell survival. Ephrins are dramatically expressed in a wide range of regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.Overexpression of B-type ephrin in cancer cells is reported to correlate with high invasion and high vascularity of tumors and elevated expression of ephrin-B1 is observed in poorly differentiated invasive tumor cells and other tumors with poor clinical prognosis4.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1

Short Description :

A Rabbit Polyclonal Antibody to Ephrin-B1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Ephrin-B1. Anti-Ephrin-B1 (extracellular) Antibody (#AER-041) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the ligand in living cells. It has been designed to recognize Ephrin-B1 from human, rat and mouse samples.

Negative Control :

BLP-ER041

Positive Control :

Synonyms :

EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EFNB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage