Anti-Ephrin-A5 (extracellular) Antibody | Alomone Labs AER-035 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ephrin-A5 (extracellular) Antibody

catalog :

AER-035

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).3,4. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).

image 2 :

Expression of Ephrin-A5 rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Ephrin A5 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (arrows) and in the granule layer (GL). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AER-035

SKU :

AER-035-CF_0.2 ml

Product Name :

Anti-Ephrin-A5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P52803

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER035

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5

Product Page - Scientific background :

The Eph family of tyrosine kinase receptor signaling pathway is important for a variety of physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1-3.Eph receptors consist of two large subfamilies: EphA and EphB that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Ephrin ligands are divided into ephrin-A (1 to 5) and ephrin-B (1 to 3)1,2.Ephrin-A5 is predominantly expressed in the quiescent anterior lens epithelial cells and is highly expressed in different layers of the cortex2,3.Studies show that mutations in ephrin-A5 are linked to congenital and age-related cataracts in humans and mice. Loss of ephrin-A5 causes abnormal localization of E-cadherin and β-catenin in anterior epithelial cells and epithelial-mesenchymal-transition2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

EPH-related receptor tyrosine kinase ligand 7, LERK7, AL-1, EFNA5

Short Description :

A Rabbit Polyclonal Antibody to Ephrin A5

Long Description :

Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) is a highly specific antibody directed against an epitope of human EFNA5. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize EFNA5 from rat, mouse, and human samples.

Negative Control :

BLP-ER035

Positive Control :

Synonyms :

EPH-related receptor tyrosine kinase ligand 7, LERK7, AL-1, EFNA5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EFNA5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage