Anti-Ephrin-A2 (extracellular) Antibody | Alomone Labs AER-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ephrin-A2 (extracellular) Antibody

catalog :

AER-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Ephrin-A2 (extracellular) Antibody (#AER-032), (1:400).3,4. Anti-Ephrin-A2 (extracellular) Antibody, preincubated with Ephrin-A2 (extracellular) Blocking Peptide (#BLP-ER032).

image 2 :

Expression of Ephrin-A2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Ephrin-A2 (extracellular) Antibody (#AER-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. Ephrin-A2 staining (green) in hippocampal dentate gyrus appears in neurons (vertical arrows) and astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AER-032

SKU :

AER-032-CF_0.2 ml

Product Name :

Anti-Ephrin-A2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P52801

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER032

Homology :

Rat - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2

Product Page - Scientific background :

Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1-A8 and A10) and EphB (B1-B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins. The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2

Short Description :

A Rabbit Polyclonal Antibody to Ephrin-A2

Long Description :

Anti-Ephrin-A2 (extracellular) Antibody (#AER-032) is a highly specific antibody directed against an epitope of mouse EFNA2. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize EFNA2 from rat, mouse, and human samples.

Negative Control :

BLP-ER032

Positive Control :

Synonyms :

EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EFNA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage