Anti-Ephrin-A1 (extracellular) Antibody | Alomone Labs AER-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ephrin-A1 (extracellular) Antibody

catalog :

AER-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Takasugi M, Okada R, Takahashi A, Virya Chen D, Watanabe S, Hara E. Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2. Nat Commun. 2017;8:15729 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and human Malme-3M melanoma cell lysate (lanes 2 and 4): - 1,2. Anti-Ephrin-A1 (extracellular) Antibody (#AER-031), (1:200).3,4. Anti-Ephrin-A1 (extracellular) Antibody, preincubated with Ephrin-A1 (extracellular) Blocking Peptide (#BLP-ER031).

image 2 :

Expression of Ephrin-A1 in rat PC12 cells - Cell surface detection ofEphrin-A1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-Ephrin-A1 (extracellular) Antibody (#AER-031) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.

image 3 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and human Malme-3M melanoma cell lysate (lanes 2 and 4): - 12.Anti-Ephrin-A1 (extracellular) Antibody (#AER-031) (1:200).34. Anti-Ephrin-A1 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AER-031

SKU :

AER-031-CF_0.2 ml

Product Name :

Anti-Ephrin-A1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20827

Applications :

IC IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER031

Homology :

Mouse, rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KITHSPQAHDNPQEK, corresponding to amino acid residues 150-164 of human Ephrin-A1

Product Page - Scientific background :

Ephrin-A1, a ligand of type-A Eph receptor tyrosine kinases and the Eph/ephrin system, has been shown to play important roles in the nervous system, tissue patterning and cell adhesion through receptor-ligand interactions and intracellular signaling1. Ephrins can be divided into two subclasses: ephrin A and ephrin B. Ephrin A ligands (ephrin A1-A5) are anchored to the cell surface via a glycosylphosphatidylinositol (GPI) anchor and bind to Eph A receptors (Eph A1-A8), whereas ephrin B ligands (ephrin B1-B3) have transmembrane and cytoplasmic domains and interact with Eph B receptors (Eph B1-B6)2.Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they have well-known roles in the establishment of neuronal connectivity by guiding axons to the appropriate targets and regulating the formation of synaptic connections. Ephrins are expressed in numerous regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.Ephrin (Eph) receptors are frequently overexpressed in a variety of human malignant tumors, and are associated with tumor growth, invasion, metastasis and angiogenesis. Recent studies using clinical samples showed that ephrin-A1 expression was up-regulated in colorectal cancer and hepatocellular carcinoma and positively correlated with a poor prognostic value4.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

EPH-related receptor tyrosine kinase ligand 1, LERK1, EFNA1

Short Description :

A Rabbit Polyclonal Antibody to Ephrin-A1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human Ephrin-A1. Anti-Ephrin-A1 (extracellular) Antibody (#AER-031) can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting Ephrin-A1 in living cells. It has been designed to recognize Ephrin-A1 from human, rat and mouse samples.

Negative Control :

BLP-ER031

Positive Control :

Synonyms :

EPH-related receptor tyrosine kinase ligand 1, LERK1, EFNA1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EFNA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage