Anti-EphB1 (extracellular) Antibody | Alomone Labs AER-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-EphB1 (extracellular) Antibody

catalog :

AER-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Pentz R, Iulita M, Ducatenzeiler A, Videla L, Benejam B, Carmona Iragui M, et al. Nerve growth factor (NGF) pathway biomarkers in Down syndrome prior to and after the onset of clinical Alzheimer's disease: A paired CSF and plasma study. Alzheimers Dement. 2021;17:605-617 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-EphB1 (extracellular) Antibody (#AER-021), (1:800).3,4. Anti-EphB1 (extracellular) Antibody, preincubated with EphB1 (extracellular) Blocking Peptide (#BLP-ER021).

image 2 :

Cell surface detection of EphB1 in live intact human THP-1 monocytic leukemia cell line: - ___Cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.___Cells +Anti-EphB1 (extracellular) Antibody (#AER-021) (1:15) + goat-anti-rabbit-Alexa-488.

image 3 :

Expression of EphB1 in rat PC12 cells - Cell surface detection ofEphB1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-EphB1 (extracellular) Antibody (#AER-021) (1:100) followed by goat anti-rabbit-AlexaFluor-594(red). B. Live image of the cells. C. Merge of the two images.

product information

CAT :

AER-021

SKU :

AER-021-CF_0.2 ml

Product Name :

Anti-EphB1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CBF3

Applications :

IC IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER021

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RSQTNTARIDGLR, corresponding to amino acid residues 485-497 of mouse EphB1

Product Page - Scientific background :

Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer's disease1.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2

Short Description :

A Rabbit Polyclonal Antibody to EphB1 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse EphB1. Anti-EphB1 (extracellular) Antibody (#AER-021) can be used in western blot, immunocytochemistry and indirect flow cytometry applications. The antibody is suitable for live cell imaging. It has been designed to recognize EphB1 from rat, mouse, and human samples.

Negative Control :

BLP-ER021

Positive Control :

Synonyms :

Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EPHB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage