Anti-EphA6 (extracellular) Antibody | Alomone Labs AER-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-EphA6 (extracellular) Antibody

catalog :

AER-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1, 2. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200). 3, 4. Anti-EphA6 (extracellular) Antibody, preincubated with EphA6 (extracellular) Blocking Peptide (#BLP-ER016).

image 2 :

Expression of EphA6 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200), followed by goat anti-rabbit-AlexaFluor-488. EphA6 immunoreactivity (green) appears in pyramidal cells (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AER-016

SKU :

AER-016-CF_0.2 ml

Product Name :

Anti-EphA6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P54758

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER016

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6

Product Page - Scientific background :

EphA6 is an erythropoietin-producing hepatocellular (Eph) receptor that belongs to the tyrosine kinase receptor family. The Eph receptor family consists of 9 EphA receptors and 5 EphB receptors, which serve key roles in normal physiology, including cell migration, cell adhesion, cell proliferation and neuronal synaptic plasticity. These activities depend on the binding of their Eph receptor-interacting (ephrin) proteins, of which include 8 members. Compelling data has demonstrated that Eph receptors and ephrins affect tumor growth, invasiveness, angiogenesis and metastasis1. They are also involved in brain development, learning and memory2.EphA6 signaling pathways are inhibitory for developing axons and blocking these pathways enhances regeneration following spinal cord and brain injury; rendering EphA6 an appealing drug target3.Eph receptors contain an extracellular structure consisting of an ephrin binding domain, the juxtamembrane region connects the extracellular portion of the receptor to the intracellular kinase domain. Two Tyrosine residues on the juxtamembrane region mediate autophosphorylation and receptor dimerization mediates the formation of heterocomplexes that are required for signaling4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2

Short Description :

A Rabbit Polyclonal Antibody to EphA6 Receptor

Long Description :

Anti-EphA6 (extracellular) Antibody (#AER-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize EphA6 from rat, mouse, and human samples.

Negative Control :

BLP-ER016

Positive Control :

Synonyms :

Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EPHA6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage