Anti-EphA1 (extracellular) Antibody | Alomone Labs AER-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-EphA1 (extracellular) Antibody

catalog :

AER-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-EphA1 (extracellular) Antibody (#AER-011), (1:200).3,4. Anti-EphA1 (extracellular) Antibody, preincubated with EphA1 (extracellular) Blocking Peptide (#BLP-ER011).

image 2 :

Expression of EphA1 in rat striatum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA1 (extracellular) Antibody (#AER-011), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. EphA1 immunoreactivity (red) appears in striatal neurons (arrows). B. Pre-incubation of the antibody with EphA1 (extracellular) Blocking Peptide (#BLP-ER011), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of human Jurkat T cell leukemia cells (lanes 1 and 5) human HeLa cervix adenocarcinoma cells (lanes 2 and 6) human SH-SY5Y neuroblastoma cells (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cells (lanes 4 and 8): - 1-4.Anti-EphA1 (extracellular)Antibody (#AER-011) (1:200).5-8. Anti-EphA1 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AER-011

SKU :

AER-011-CF_0.2 ml

Product Name :

Anti-EphA1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21709

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER011

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1

Product Page - Scientific background :

Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer's disease1.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1

Short Description :

A Rabbit Polyclonal Antibody to EphA1 Receptor

Long Description :

Anti-EphA1 (extracellular) Antibody (#AER-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize EphA1 from rat, mouse, and human samples.

Negative Control :

BLP-ER011

Positive Control :

Synonyms :

Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EPHA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage