Anti-Endothelin Receptor A (extracellular) Antibody | Alomone Labs AER-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Endothelin Receptor A (extracellular) Antibody

catalog :

AER-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of mouse lung lysate:

image 3 :

Western blot analysis of human HUVEC endothelial cell line lysate (lanes 1 and 4), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 5) and human U-87 MG glioblastoma cell line lysate (lanes 3 and 6):

product information

CAT :

AER-004

SKU :

AER-004-CF_0.2 ml

Product Name :

Anti-Endothelin Receptor A (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P26684

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER004

Homology :

Mouse - identical Human - 12 out of 13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KKTVYDEMDKNRC, corresponding to amino acid residues 329 - 341 of rat EDNRA

Product Page - Scientific background :

The endothelin system is comprised of three active peptides, ET-1, 2, and 3, which are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein Coupled Receptors, ETAR and ETBR. Both ETAR and ETBR are present in heart and in human myocardium at similar levels.1,2The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities.Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3Overexpression of ET-1 and ETAR was reported in different malignancies including prostate cancer human Kaposi's sarcoma, ovarian and breast carcinomas.4In breast carcinomas overexpression of ET-1 and ETA receptors correlated with parameters that characterize aggressive types of breast cancer suggesting that analysis of ETAR expression might be used as a diagnostic marker for evaluating the progression of the disease and effectiveness of treatment. These and other findings have made ET receptors, and especially ETAR, promising therapeutic targets for pharmacological intervention.5-9

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

EDNRA, ET-A, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor, Endothelin Receptor Type A

Short Description :

A Rabbit Ployclonal Antibody to Endothelin Receptor A

Long Description :

Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize ET-A Receptor from mouse, rat and human samples.

Negative Control :

BLP-ER004

Positive Control :

Synonyms :

EDNRA, ET-A, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor, Endothelin Receptor Type A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EDNRA

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage