Anti-Urotensin II Receptor Antibody | Alomone Labs AER-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Urotensin II Receptor Antibody

catalog :

AER-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Caroccia B, Menegolo M, Seccia T, Petrelli L, Antonello M, Limena A, et al. Urotensin II Exerts Pressor Effects By Stimulating Renin And Aldosterone Synthase Gene Expression. Sci Rep. 2017;7:13876 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-Urotensin II Receptor Antibody (#AER-003), (1:200). 2. Anti-Urotensin II Receptor Antibody, preincubated with Urotensin II Receptor Blocking Peptide (#BLP-ER003).

image 2 :

Western blot analysisof rat kidney membranes: - 1.Anti-UrotensinII ReceptorAntibody (#AER-003) (1:200). 2. Anti-Urotensin II Receptor Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)GRYMGDRSIPHKK corresponding to amino acid residues 413-425 of rat GABRB3 (Accession P63079). 2nd intracellular loop.

product information

CAT :

AER-003

SKU :

AER-003-CF_0.2 ml

Product Name :

Anti-Urotensin II Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49684

Applications :

IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER003

Homology :

Bovine - identical; human, canine - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RPLDTVQRSKGYRK, corresponding to amino acid residues 154-167 of rat URIIR

Product Page - Scientific background :

Urotensin II is a powerful vasoconstrictive substance. The human urotensin II receptor (UR-II-R) was originally isolated as an orphan receptor (GPR14) from neural and sensory tissues.1,2 Urotensin II is expressed in various human tissues such as the CNS, skeletal muscle, smooth muscle, pancreas, and heart.3-5 Binding of urotensin II to the receptor leads to activation of phospholipase C. The resulting increase in intracellular Ca2+ may cause the contraction of vascular smooth muscle. ERK1/2 phophorylation may also be involved in mediating the biological activities of urotensin II.6 Recently, evidence has accumulated indicating that urotensin II is also expressed in various kinds of tumor cells such as adrenal tumors and neuroblastomas. In those cells, urotensin II was reported to stimulate cell growth.7-9

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Urotensin 2 receptor, UR-II-R, UR2R, UTS2R, SENR, GPR14

Short Description :

A Rabbit Ployclonal Antibody to Urotensin II Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against the 2nd cytoplasmic loop of the rat UTS2R. Anti-Urontenisn II Receptor Antibody (#AER-003) can be used in western blot and immunohistochemical applications, and recognizes UTS2R in rat samples.

Negative Control :

BLP-ER003

Positive Control :

Synonyms :

Urotensin 2 receptor, UR-II-R, UR2R, UTS2R, SENR, GPR14

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

UTS2R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage