Anti-Endothelin Receptor B Antibody | Alomone Labs AER-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Endothelin Receptor B Antibody

catalog :

AER-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Reference
Abdul Y, Jamil S, He L, Li W, Ergul A. Endothelin-1 (ET-1) promotes a proinflammatory microglia phenotype in diabetic conditions. Can J Physiol Pharmacol. 2020;98:596-603 pubmed publisher
Gupta S, Prajapati A, Gulati M, Gautam S, Kumar S, Dalal V, et al. Irreversible and sustained upregulation of endothelin axis during oncogene-associated pancreatic inflammation and cancer. Neoplasia. 2020;22:98-110 pubmed publisher
Guil M, Sch ller M, Cassinotti L, Biancardi V, Pitra S, Bianciotti L, et al. Role of endothelin receptor type A on catecholamine regulation in the olfactory bulb of DOCA-salt hypertensive rats: Hemodynamic implications. Biochim Biophys Acta Mol Basis Dis. 2019;1865:165527 pubmed publisher
Krawczyk K, Skovsted G, Perisic L, Dreier R, Berg J, Hedin U, et al. Expression of endothelin type B receptors (EDNRB) on smooth muscle cells is controlled by MKL2, ternary complex factors and actin dynamics. Am J Physiol Cell Physiol. 2018;: pubmed publisher
Ramkumar N, Stuart D, Abraham N, Kohan D. Nephron prorenin receptor deficiency alters renal medullary endothelin-1 and endothelin receptor expression. Physiol Res. 2018;67:S127-S136 pubmed
Armando I, Asico L, Wang X, Jones J, Serrão M, Cuevas S, et al. Antihypertensive effect of etamicastat in dopamine D2 receptor-deficient mice. Hypertens Res. 2018;41:489-498 pubmed publisher
Skovsted G, Kruse L, Berchtold L, Grell A, Warfvinge K, Edvinsson L. Myocardial ischemia-reperfusion enhances transcriptional expression of endothelin-1 and vasoconstrictor ETB receptors via the protein kinase MEK-ERK1/2 signaling pathway in rat. PLoS ONE. 2017;12:e0174119 pubmed publisher
Lohsiriwat V, Scholefield J, Wilson V, Dashwood M. Endothelin-1 and its receptors on haemorrhoidal tissue: a potential site for therapeutic intervention. Br J Pharmacol. 2017;174:569-579 pubmed publisher
Amor S, García Villalón A, Rubio C, Carrascosa J, Monge L, Fernandez N, et al. Effects of age and caloric restriction in the vascular response of renal arteries to endothelin-1 in rats. Exp Gerontol. 2017;88:32-41 pubmed publisher
Yasir A, Hardigan T, Ergul A. Diabetes-mediated middle cerebral artery remodeling is restored by linagliptin: Interaction with the vascular smooth muscle cell endothelin system. Life Sci. 2016;159:76-82 pubmed publisher
Forner S, Martini A, Andrade E, Rae G. Neuropathic pain induced by spinal cord injury: Role of endothelin ETA and ETB receptors. Neurosci Lett. 2016;617:14-21 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Endothelin Receptor B Antibody (#AER-002), (1:200).2. Anti-Endothelin Receptor B Antibody, preincubated with Endothelin Receptor B Blocking Peptide (#BLP-ER002).

image 2 :

Expression of EDNRB in rat lung - Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor B Antibody (#AER-002) (1:100). EDNRB is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Note that vascular smooth muscle is not stained (blue arrows). Hematoxilin is used as the counterstain.

image 3 :

Peptide CEMLRKKSGMQIALND corresponding to amino acid residues 298-314 of rat ET-B (AccessionP21451). 3rdintracellularloop.

product information

CAT :

AER-002

SKU :

AER-002-CF_0.2 ml

Product Name :

Anti-Endothelin Receptor B Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21451

Applications :

IC IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER002

Homology :

All known vertebrate sequences - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CEMLRKKSGMQIALND, corresponding to amino acid residues 298-314 of rat ET-B

Product Page - Scientific background :

Endothelins (ET-1, 2, and 3) are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-protein coupled receptors, ETAR and ETBR. Both ETAR and ETBR are present in the heart and in human myocardium at similar levels.1,2The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 > ET-2 > ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular, as well as in non-vascular, cells and tissues, having multiple biological activities.Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3Until recently, it was thought that all cellular activities of the endothelins were mediated through their interactions with their cell surface receptors. However, a recent study demonstrated that cardiac nuclei also possess both ETAR and ETBR subtypes, which are functional and coupled to signaling mechanisms within the nuclear membrane.2Hypermethylation of the ETBR correlated with transcriptional down-regulation and reduced expression of ETB receptor was observed in several prostate, bladder and colon cancer cell lines.3

Applications may also work in :

IC IF IFC IHC WB

Supplier :

Alomone Labs

Target :

EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type

Short Description :

A Rabbit Polyclonal Antibody to Endothelin Receptor B

Long Description :

Anti-Endothelin Receptor B Antibody (#AER-002) is a highly specific antibody directed against an epitope of rat ET-B. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize ET-B from rat, mouse, and human samples.

Negative Control :

BLP-ER002

Positive Control :

Synonyms :

EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EDNRB

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage