Anti-Endothelin Receptor A Antibody | Alomone Labs AER-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Endothelin Receptor A Antibody

catalog :

AER-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Abdul Y, Li W, Vargas J, Grant E, He L, Jamil S, et al. Diabetes-related sex differences in the brain endothelin system following ischemia in vivo and in human brain endothelial cells in vitro. Can J Physiol Pharmacol. 2020;98:587-595 pubmed publisher
Gupta S, Prajapati A, Gulati M, Gautam S, Kumar S, Dalal V, et al. Irreversible and sustained upregulation of endothelin axis during oncogene-associated pancreatic inflammation and cancer. Neoplasia. 2020;22:98-110 pubmed publisher
Ramkumar N, Stuart D, Abraham N, Kohan D. Nephron prorenin receptor deficiency alters renal medullary endothelin-1 and endothelin receptor expression. Physiol Res. 2018;67:S127-S136 pubmed
Subramanian M, MohanKumar S, Balasubramanian P, Northcott C, Garver H, Fink G, et al. Chronic exposure to low doses of estradiol-17ß increases blood pressure in young female rats: A possible role for central Endothelin-1. Sci Rep. 2017;7:139 pubmed publisher
Amor S, García Villalón A, Rubio C, Carrascosa J, Monge L, Fernandez N, et al. Effects of age and caloric restriction in the vascular response of renal arteries to endothelin-1 in rats. Exp Gerontol. 2017;88:32-41 pubmed publisher
Forner S, Martini A, Andrade E, Rae G. Neuropathic pain induced by spinal cord injury: Role of endothelin ETA and ETB receptors. Neurosci Lett. 2016;617:14-21 pubmed publisher
BrianÃ§on Marjollet A, Monneret D, Henri M, Hazane Puch F, Pepin J, Faure P, et al. Endothelin regulates intermittent hypoxia-induced lipolytic remodelling of adipose tissue and phosphorylation of hormone-sensitive lipase. J Physiol. 2016;594:1727-40 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Endothelin Receptor A Antibody (#AER-001), (1:200).2. Anti-Endothelin Receptor A Antibody, preincubated with Endothelin Receptor A Blocking Peptide (#BLP-ER001).

image 2 :

Expression of ET-A in rat lung - Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor A Antibody (#AER-001), (1:100). ET-A is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of ET-A in rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-Endothelin Receptor AAntibody (#AER-001) (A)and Anti-Endothelin Receptor A Antibody preincubated with the control peptide antigen (B).Cresyl violet is used as the counterstain.

product information

CAT :

AER-001

SKU :

AER-001-CF_0.2 ml

Product Name :

Anti-Endothelin Receptor A Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P26684

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ER001

Homology :

Human, swine, bovine - 13/14 amino acid residues identical; chicken - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A

Product Page - Scientific background :

The endothelin system is comprised of three active peptides, ET-1, 2, and 3, which are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein Coupled Receptors, ETAR and ETBR. Both ETAR and ETBR are present in heart and in human myocardium at similar levels.1,2The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities.Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3Overexpression of ET-1 and ETAR was reported in different malignancies including prostate cancer human Kaposi's sarcoma, ovarian and breast carcinomas.4In breast carcinomas overexpression of ET-1 and ETA receptors correlated with parameters that characterize aggressive types of breast cancer suggesting that analysis of ETAR expression might be used as a diagnostic marker for evaluating the progression of the disease and effectiveness of treatment. These and other findings have made ET receptors, and especially ETAR, promising therapeutic targets for pharmacological intervention.5-9

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor

Short Description :

A Rabbit Polyclonal Antibody to Endothelin Receptor A

Long Description :

Anti-Endothelin Receptor A Antibody (#AER-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ET-AR from rat, mouse, and human samples.

Negative Control :

BLP-ER001

Positive Control :

Synonyms :

EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

EDNRA

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage