Anti-DARPP32 Antibody | Alomone Labs ADR-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-DARPP32 Antibody

catalog :

ADR-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200). 3, 4. Anti-DARPP32 Antibody, preincubated with DARPP32 Blocking Peptide (#BLP-DR006).

image 2 :

Expression of DARPP32 in rat striatum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DARPP32 Antibody (#ADR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. DARPP32 immunoreactivity (green) appears in striatal cells (arrows). ST=striatum, CC=corpus callosum. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ADR-006

SKU :

ADR-006-CF_0.2 ml

Product Name :

Anti-DARPP32 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UD71

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-DR006

Homology :

Mouse, rat - 13 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EGPWERPPPLDESER, corresponding to amino acid residues 160 - 174 of human DARPP32

Product Page - Scientific background :

Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32), also known as, Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) is a protein regulated by the phosphorylation of cAMP1.DARPP32 is expressed in the spiny neurons of the neostriatum and in the adrenal medulla, kidney, and parathyroid cells and it is also overexpressed in tumor cells from breast, colon, esophagus, lung and prostate tissues1,2. Its protein structure is comprised of 18% alpha helix, 23% beta strand, 30% beta turn, and 29% random coil3.DARPP32 has been shown to play a key role in mediating the biochemical, electrophysiological, and behavioral effects of dopamine on dopaminoceptive neurons4,5. It is also involved in mediating the actions of other neurotransmitter systems such as serotonin and glutamate, in response to a variety of drugs5,6.DARPP32 is reported to have a role in development and neurodegenerative diseases. In addition, DARPP32 plays a role in cancer tumorigenesis. Collectively, findings from published reports suggest DARPP32 as a potential biomarker of drug resistance and a therapeutic target in gastric cancer and other malignancies5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Dopamine and cAMP-regulated neuronal phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PPP1R1B

Short Description :

A Rabbit Polyclonal Antibody to DARPP32

Long Description :

Anti-DARPP32 Antibody (#ADR-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DARPP32 from human, rat, and mouse samples.

Negative Control :

BLP-DR006

Positive Control :

Synonyms :

Dopamine and cAMP-regulated neuronal phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PPP1R1B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PPP1R1B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage