Anti-D5 Dopamine Receptor (extracellular) Antibody | Alomone Labs ADR-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-D5 Dopamine Receptor (extracellular) Antibody

catalog :

ADR-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; 1:100; loading ...; fig 1e western blot; rat; 1:200; loading ...; fig 4c immunohistochemistry - frozen section; human; 1:100; loading ...; fig 2a	Feng X, Zhang D, Wang Y, Fan R, Hong F, Zhang Y, et al. Dopamine enhances duodenal epithelial permeability via the dopamine D5 receptor in rodent. Acta Physiol (Oxf). 2017;220:113-123 pubmed publisher
	Prado C, Osorio Barrios F, Falc xf3 n P, Espinoza A, Saez J, Yuseff M, et al. Dopaminergic stimulation leads B-cell infiltration into the central nervous system upon autoimmunity. J Neuroinflammation. 2021;18:292 pubmed publisher
	Liu L, Wu Y, Wang B, Jiang Y, Lin L, Li X, et al. DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization. Cell Death Dis. 2021;12:500 pubmed publisher
	Kikuta S, Iguchi Y, Kakizaki T, Kobayashi K, Yanagawa Y, Takada M, et al. Store-Operated Calcium Channels Are Involved in Spontaneous Slow Calcium Oscillations in Striatal Neurons. Front Cell Neurosci. 2019;13:547 pubmed publisher
	Mueller A, Shepard S, Moore T. Differential Expression of Dopamine D5 Receptors across Neuronal Subtypes in Macaque Frontal Eye Field. Front Neural Circuits. 2018;12:12 pubmed publisher
	Zhang L, Hernandez V, Swinny J, Verma A, Giesecke T, Emery A, et al. A GABAergic cell type in the lateral habenula links hypothalamic homeostatic and midbrain motivation circuits with sex steroid signaling. Transl Psychiatry. 2018;8:50 pubmed publisher
	Tekko T, Lakspere T, Allikalt A, End J, Kõlvart K, Jagomäe T, et al. Wfs1 is expressed in dopaminoceptive regions of the amniote brain and modulates levels of D1-like receptors. PLoS ONE. 2017;12:e0172825 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain membranes: - 1. Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005), (1:200).2. Anti-D5 Dopamine Receptor (extracellular) Antibody, preincubated with D5 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR005).

image 2 :

Expression of D5 dopamine receptor in rat striatum - Immunohistochemical staining of perfusion-fixed rat brain frozen using Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005), (1:100). A. DRD5 (green) appears in a subset of striatal neurons and in the striatal matrix. B. Staining of the same section with calbindin D28k (red), a marker of interneurons. C. Merging the two images demonstrates that DRD5 partially overlaps with the population of calbindin containing striatal interneurons. DAPI is used as the counterstain (blue).

image 3 :

Peptide EEGWELEGRTENC corresponding to amino acid residues 199-211 of rat DRD5(AccessionP25115). 2ndextracellular loop.

product information

CAT :

ADR-005

SKU :

ADR-005-CF_0.2 ml

Product Name :

Anti-D5 Dopamine Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25115

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-DR005

Homology :

Mouse - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

EEGWELEGRTENC, corresponding to amino acid residues 199-211 of rat DRD5

Product Page - Scientific background :

The D5 dopamine receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and Schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery, the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract.The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knockout mice have shown no overt alterations in locomotor or cognitive functions. However, knockout mice do develop severe hypertension suggesting a role of the D5 receptor in the modulation of neuronal pathways regulating blood pressure responses.2,3

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

DRD5, D(1B) dopamine receptor

Short Description :

A Rabbit Polyclonal Antibody to D5 Dopamine Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 2nd extracellular loop of the rat D5 dopamine receptor. Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005) can be used in western blot analysis and immunohistochemical applications, and recognizes DRD5 from rat and mouse samples.

Negative Control :

BLP-DR005

Positive Control :

Synonyms :

DRD5, D(1B) dopamine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DRD5

AB Specifictiy :

Unlikely to recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage