Anti-D4 Dopamine Receptor Antibody | Alomone Labs ADR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-D4 Dopamine Receptor Antibody

catalog :

ADR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; tbl 3	Higa K, Grim A, Kamenski M, van Enkhuizen J, Zhou X, Li K, et al. Nicotine withdrawal-induced inattention is absent in alpha7 nAChR knockout mice. Psychopharmacology (Berl). 2017;234:1573-1586 pubmed publisher
	Kim M, Yu H, Ju H, Shin J, Kim A, Lee J, et al. Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels. Sci Rep. 2019;9:16328 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-D4 Dopamine Receptor Antibody (#ADR-004), (1:200).3,4. Anti-D4 Dopamine Receptor Antibody, preincubated with D4 Dopamine Receptor Blocking Peptide (#BLP-DR004).

image 2 :

Peptide (C)RRWEAARHTKLHSR corresponding to amino acid residues 215-228 of rat DRD4(AccessionP30729). 3rdintracellular loop.

image 3 :

Expression of 5-Hydroxytryptamine receptor 1B in rat and mouse brain - Immunohistochemical staining of rat and mouse brain sections using Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO-488 Antibody(#ASR-022-AG) (1:80). A. 5-HT1B receptor staining (green) in rat hippocampal CA1 region is detected near the pyramidal layer (P) and in apical dendrites (arrows). B. In mouse hippocampal CA1 region 5-HT1B receptor staining (green) appears in the pyramidal layer (P) and in apical dendrites. Nuclei are stained using DAPI (blue).

product information

CAT :

ADR-004

SKU :

ADR-004-CF_0.2 ml

Product Name :

Anti-D4 Dopamine Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30729

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-DR004

Homology :

Mouse - identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4

Product Page - Scientific background :

The dopamine neurotransmitter belongs to the catecholamines and can therefore be further converted into adrenaline and noreadrenaline. Dopamine has various physiological roles, including learning and memory, motor output and endocrine regulation. It does so by binding and activating dopamine receptors which belong to the G-protein coupled receptor superfamily (GPCR)1.The D4 dopamine receptor (DRD4) belongs to the D2-like family as do D2 and D3 dopamine receptors. Like all GPCRs, it has seven transmembrane spanning membrane regions. Structure-wise, members of the family share high homology in the transmembrane domains and lower homology in the extracellular N-terminal and the intracellular C-terminal domains. Notably, the coding region of the 3rd intracellular loop of DRD4 is known to undergo extensive polymorphism2.Like many GPCRs, each dopamine receptor subtype can react with more than one G-protein giving rise to different signaling possibilities3. Whereas D2-like dopamine receptors are generally considered to couple to Gi, and therefore inhibit adenylyl cyclase, the signaling through DRD4 is complicated due to the polymorphisms in the 3rd intracellular loop. It seems that this region is important to G-coupling as different polyphormisms in the region influence the ability of DRD4 to couple to adenylyl cyclase and G-proteins4,5. D4 dopamine receptors also influence Ca2+ levels3,6. They could also interact with G-protein couple inwardly rectifying K+ channel to ultimately cause a decrease in the firing rate of neurons7.The distribution of DRD4 mostly includes the brain and is mainly found post-synaptically in dendritic shafts and spines of mammalian striatum8.

Applications may also work in :

Supplier :

Alomone Labs

Target :

DRD4, D4DR, D(2C) dopamine receptor

Short Description :

A Rabbit Polyclonal Antibody to D4 Dopamine Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 3rd intracellular loop of the rat D4 dopamine receptor. Anti-D4 Dopamine Receptor Antibody (#ADR-004) can be used in western blot analysis, and recognizes DRD4 from rat and mouse samples.

Negative Control :

BLP-DR004

Positive Control :

Synonyms :

DRD4, D4DR, D(2C) dopamine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DRD4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage