Anti-D3 Dopamine Receptor (extracellular) Antibody | Alomone Labs ADR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-D3 Dopamine Receptor (extracellular) Antibody

catalog :

ADR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Prado C, Osorio Barrios F, Falc xf3 n P, Espinoza A, Saez J, Yuseff M, et al. Dopaminergic stimulation leads B-cell infiltration into the central nervous system upon autoimmunity. J Neuroinflammation. 2021;18:292 pubmed publisher
Montoya A, Elgueta D, Campos J, Chovar O, Falc xf3 n P, Matus S, et al. Dopamine receptor D3 signalling in astrocytes promotes neuroinflammation. J Neuroinflammation. 2019;16:258 pubmed publisher
Olingy C, San Emeterio C, Ogle M, Krieger J, Bruce A, Pfau D, et al. Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury. Sci Rep. 2017;7:447 pubmed publisher
Sol s O, Garcia Montes J, Gonz lez Granillo A, Xu M, Moratalla R. Dopamine D3 Receptor Modulates l-DOPA-Induced Dyskinesia by Targeting D1 Receptor-Mediated Striatal Signaling. Cereb Cortex. 2017;27:435-446 pubmed publisher

image

image 1 :

Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes: - 1,2. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).3,4. Anti-D3 Dopamine Receptor (extracellular) Antibody, preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).

image 2 :

Expression of DRD3 in rat striatum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:100). A. DRD3 appears in a subset of striatal neurons (green). B. The same section was stained for parvalbumin (red), a marker of interneurons. C. Merging the two images demonstrates that DRD3 localization includes parvalbuminergic striatal interneurons. DAPI was used as the counterstain (blue).

image 3 :

Peptide CGAENSTGVNRARPH corresponding to amino acid residues 15-29 of rat DRD3(AccessionP19020). Extracellular N-terminus.

product information

CAT :

ADR-003

SKU :

ADR-003-CF_0.2 ml

Product Name :

Anti-D3 Dopamine Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19020

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-DR003

Homology :

Mouse - identical; human - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat DRD3

Product Page - Scientific background :

The D3 Dopamine Receptor (D3 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1The D3 receptor distribution in the brain is relatively restricted to limbic areas such as striatum, islands of Calleja and olfactory tubercle. In the periphery, it is expressed in the kidney particularly in proximal tubules.1The exact physiological function of the D3 receptor remains to be fully elucidated. In studies using D3 receptor knockout mice, the most prominent dysfunction was the development of rennin-dependent hypertension. Potential roles in reinforcement and reward behaviors have also been suggested as well as roles in neuropsychiatric disorders such as drug abuse and schizophrenia.2

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

DRD3

Short Description :

A Rabbit Polyclonal Antibody to D3 Dopamine Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus of the rat D3 dopamine receptor. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003) can be used in western blot analysis and immunohistochemical applications, and recognizes DRD3 from rat and mouse samples.

Negative Control :

BLP-DR003

Positive Control :

Synonyms :

DRD3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DRD3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage