Anti-D2 Dopamine Receptor (extracellular) Antibody | Alomone Labs ADR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-D2 Dopamine Receptor (extracellular) Antibody

catalog :

ADR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Prado C, Osorio Barrios F, Falc xf3 n P, Espinoza A, Saez J, Yuseff M, et al. Dopaminergic stimulation leads B-cell infiltration into the central nervous system upon autoimmunity. J Neuroinflammation. 2021;18:292 pubmed publisher
Sadamura Y, Thapa S, Mizunuma R, Kambe Y, Hirasawa A, Nakamoto K, et al. FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity in Mice. Front Pharmacol. 2021;12:699026 pubmed publisher
Chang C, Lo Y, Lin S, Yang S, Lin H, Lin T, et al. Modulation of Theta-Band Local Field Potential Oscillations Across Brain Networks With Central Thalamic Deep Brain Stimulation to Enhance Spatial Working Memory. Front Neurosci. 2019;13:1269 pubmed publisher
Zhou L, Ran X, Hong F, Li G, Zhu J. Downregulated Dopamine Receptor 2 and Upregulated Corticotrophin Releasing Hormone in the Paraventricular Nucleus Are Correlated With Decreased Glucose Tolerance in Rats With Bilateral Substantia Nigra Lesions. Front Neurosci. 2019;13:751 pubmed publisher
Pelassa S, Guidolin D, Venturini A, Averna M, Frumento G, Campanini L, et al. A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence. Int J Mol Sci. 2019;20: pubmed publisher
Yang Y, Ran X, Li Y, Zhou L, Zheng L, Han Y, et al. Expression of Dopamine Receptors in the Lateral Hypothalamic Nucleus and Their Potential Regulation of Gastric Motility in Rats With Lesions of Bilateral Substantia Nigra. Front Neurosci. 2019;13:195 pubmed publisher
Hasbi A, Perreault M, Shen M, Fan T, Nguyen T, Alijaniaram M, et al. Activation of Dopamine D1-D2 Receptor Complex Attenuates Cocaine Reward and Reinstatement of Cocaine-Seeking through Inhibition of DARPP-32, ERK, and ΔFosB. Front Pharmacol. 2017;8:924 pubmed publisher
Areal L, Herlinger A, Pelição F, Martins Silva C, Pires R. Crack cocaine inhalation induces schizophrenia-like symptoms and molecular alterations in mice prefrontal cortex. J Psychiatr Res. 2017;91:57-63 pubmed publisher
Matchynski Franks J, Susick L, Schneider B, Perrine S, Conti A. Impaired Ethanol-Induced Sensitization and Decreased Cannabinoid Receptor-1 in a Model of Posttraumatic Stress Disorder. PLoS ONE. 2016;11:e0155759 pubmed publisher

image

image 1 :

Western blot analysis of rat striatum (lanes 1 and 5) and hippocampus (lanes 2 and 6) membranes and of rat (lanes 3 and 7) and mouse (lanes 4 and 8) whole brain lysates: - 1-4. Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002), (1:200).5-8. Anti-D2 Dopamine Receptor (extracellular) Antibody, preincubated with D2 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR002).

image 2 :

Peptide (C)DDLERQNWSRPFNGSE corresponding to amino acid residues 11-26 of rat DRD2(AccessionP61169). Extracellular N-terminus.

product information

CAT :

ADR-002

SKU :

ADR-002-CF_0.2 ml

Product Name :

Anti-D2 Dopamine Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P61169

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-DR002

Homology :

Mouse - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2

Product Page - Scientific background :

The D2 dopamine receptor (DRD2) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1D2 receptor expression in the brain is largely confined to the striatum, prefrontal cortex and hypothalamus.1Dysregulation of D2 receptor function has been implicated in several disorders including schizophrenia, bipolar disorder, Parkinson's disease, and restless legs syndrome. Consistent with this, treatments that either block or activate the D2 receptor have been developed to treat these diseases.2

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

DRD2, D(2) dopamine receptor

Short Description :

A Rabbit Polyclonal Antibody to D2 Dopamine Receptor

Long Description :

Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect flow cytometry applications. It has been designed to recognize DRD2 from rat, human, and mouse samples.

Negative Control :

BLP-DR002

Positive Control :

Synonyms :

DRD2, D(2) dopamine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DRD2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage