Anti-D1 Dopamine Receptor Antibody | Alomone Labs ADR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-D1 Dopamine Receptor Antibody

catalog :

ADR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Nakamori H, Noda K, Mitsui R, Hashitani H. Role of enteric dopaminergic neurons in regulating peristalsis of rat proximal colon. Neurogastroenterol Motil. 2021;33:e14127 pubmed publisher
Chen B, Yang Y, Li S, Zhu X, Qi Y, Hong F. The critical role of hippocampal dopamine in the pathogenesis of hepatic encephalopathy. Physiol Res. 2021;70:101-110 pubmed
Conley T, Beaudin S, Lasley S, Fornal C, Hartman J, Uribe W, et al. Early postnatal manganese exposure causes arousal dysregulation and lasting hypofunctioning of the prefrontal cortex catecholaminergic systems. J Neurochem. 2020;153:631-649 pubmed publisher
Zhou L, Ran X, Hong F, Li G, Zhu J. Downregulated Dopamine Receptor 2 and Upregulated Corticotrophin Releasing Hormone in the Paraventricular Nucleus Are Correlated With Decreased Glucose Tolerance in Rats With Bilateral Substantia Nigra Lesions. Front Neurosci. 2019;13:751 pubmed publisher
Yang Y, Ran X, Li Y, Zhou L, Zheng L, Han Y, et al. Expression of Dopamine Receptors in the Lateral Hypothalamic Nucleus and Their Potential Regulation of Gastric Motility in Rats With Lesions of Bilateral Substantia Nigra. Front Neurosci. 2019;13:195 pubmed publisher
Li X, Huang M, Yang L, Guo N, Yang X, Zhang Z, et al. Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of ?-Aminobutyric Acid and Dopamine Systems. Front Neurol. 2018;9:309 pubmed publisher
Stojanovic T, Orlova M, Sialana F, HÃ¶ger H, Stuchlík S, Milenkovic I, et al. Validation of dopamine receptor DRD1 and DRD2 antibodies using receptor deficient mice. Amino Acids. 2017;49:1101-1109 pubmed publisher
Tekko T, Lakspere T, Allikalt A, End J, Kõlvart K, Jagomäe T, et al. Wfs1 is expressed in dopaminoceptive regions of the amniote brain and modulates levels of D1-like receptors. PLoS ONE. 2017;12:e0172825 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:200).3,4. Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).

image 2 :

Expression of DRD1 in rat cortex - Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:100), (green). A. DRD1 appears in the soma and dendrites of cortical pyramidal neurons in layer 5. B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of DRD1 is restricted to pyramidal neurons. DAPI is used as the counterstain.

image 3 :

Peptide SSHHEPRGSISKDC corresponding to amino acid residues 372-385 of rat DRD1(AccessionP18901). Intracellular C-terminus.

product information

CAT :

ADR-001

SKU :

ADR-001-CF_0.2 ml

Product Name :

Anti-D1 Dopamine Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18901

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-DR001

Homology :

Mouse, dog, pig - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1

Product Page - Scientific background :

The D1 dopamine receptor (D1 receptor, DRD1) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and Schizophrenia1.All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1.The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1.The D1 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 receptor has been detected in the adrenal cortex, kidney and heart.Functionally, the D1 receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways.In addition, the D1 receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

DRD1, D(1A) dopamine receptor

Short Description :

A Rabbit Polyclonal Antibody to D1 Dopamine Receptor

Long Description :

Anti-D1 Dopamine Receptor Antibody (#ADR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DRD1 from rat, mouse, and human samples.

Negative Control :

BLP-DR001

Positive Control :

Synonyms :

DRD1, D(1A) dopamine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DRD1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage