Guinea pig Anti-D1 Dopamine Receptor Antibody | Alomone Labs ADR-001-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-D1 Dopamine Receptor Antibody

catalog :

ADR-001-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Crockett S, Harris M, Boatwright N, Su R, Yarboro M, Berger C, et al. Role of dopamine and selective dopamine receptor agonists on mouse ductus arteriosus tone and responsiveness. Pediatr Res. 2020;87:991-997 pubmed publisher

image

image 1 :

Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4): - 1,2. Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:400).3,4. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).

image 2 :

Expression of DRD1 in rat cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody. DRD1 staining (red) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

ADR-001-GP

SKU :

ADR-001-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-D1 Dopamine Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18901

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-DR001

Homology :

Mouse, dog, pig - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1

Product Page - Scientific background :

The D1 dopamine receptor (D1 receptor, DRD1) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and Schizophrenia1.All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1.The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1.The D1 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 receptor has been detected in the adrenal cortex, kidney and heart.Functionally, the D1 receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways.In addition, the D1 receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

DRD1, D(1A) dopamine receptor

Short Description :

A Guinea Pig Polyclonal Antibody to D1 Dopamine Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat D1 Receptor. Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP, formerly AGP-100) raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize DRD1 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-D1 Dopamine Receptor Antibody (#ADR-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-DR001

Positive Control :

Synonyms :

DRD1, D(1A) dopamine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DRD1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage