antibody

Anti-CTL1 (SLC44A1) (extracellular) Antibody

ACT-021

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ACT-021-CF_0.2 ml

Anti-CTL1 (SLC44A1) (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q8VII6

IFC LCI WB

Human Rat Mouse

Rabbit

BLP-CT021

Mouse, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, C-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)ENSRKAMKEAGKG, corresponding to amino acid residues 626-638 of rat SLC44A1

Choline transporter-like 1, CTL1, also known as SLC44A1, CD92, and CHTL1, is a member of the broader SLC44A family and is an approximately 70 kDa choline/H+-antiporter across both plasma and mitochondrial membranes1. The CTL1 protein consists of nine transmembrane (TM) domains, with an intracellular N-terminus and an extracellular C-terminus. Sequence alignment of SLC44A proteins from rats, mice, and humans reveals homology in four TM domains (TM2, TM6, TM8, and TM9), with TM8 and TM9 exhibiting the highest conservation, suggesting that these TM domains may contain critical functional regions, including potential substrate-choline binding sites. The presence of negatively charged aspartic acid (D) and glutamic acid (E) residues at the C-terminus of CTL1 strongly suggests their involvement in binding and transport of positively charged choline and protons.1CTL1-mediated choline transport is believed to be involved in phospholipid and betaine synthesis, and it may also play a role in choline transport for acetylcholine synthesis in non-neuronal cells. CTL1 exhibits an intermediate affinity for choline, with a Kmin value in the low micromolar range. CTL1 is selectively inhibited by the choline analogue hemicholinium-3 (CH-3).1,2CTL1 has been identified as a biomarker of monocytic cell differentiation, and its expression is elevated in differentiated dendritic cells. Aberrant choline metabolism is a hallmark of malignant transformations, and the activation of the CTL1 gene has been associated with anomalous cell-cycle regulators.1,2Loss of function of the CTL1 protein is implicated in neurodegenerative disease states and can lead to symptoms including ataxia, dysarthria, tremor, swallowing difficulties, and cognitive decline, as well as leukoencephalopathy and cerebellar atrophy. On a cellular level, the main effect of CTL1 deficiency is a significant reduction in choline transport, which is compensated by using membrane phosphatidylcholine as an internal source of choline. As a response to this crucial need for choline and phosphatidylcholine, there is a depletion of inner membrane phospholipids, specifically phosphatidylethanolamine and phosphatidylserine, as well as a deficiency in very long-chain fatty acids (VLCFAs). Collectively, these features represent the primary abnormality and the major causes of impaired plasma membrane and organelle function in CTL1 deficiency. 3

IFC LCI WB

Alomone Labs

Choline Transporter-Like Protein 1, Solute carrier family 44 member 1, CD92

A Rabbit Polyclonal antibody to CTL1 (SLC44A1) (extracellular)

Anti-CTL1 (SLC44A1) (extracellular) Antibody (#ACT-021) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize CTL1 from mouse, rat and human samples.

BLP-CT021

NA

Choline Transporter-Like Protein 1, Solute carrier family 44 member 1, CD92

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC44A1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200