Anti-SLC22A2 (OCT2) Antibody | Alomone Labs ACT-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC22A2 (OCT2) Antibody

catalog :

ACT-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney lysates (lanes 2 and 4): - 1-2. Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200). 3-4. Anti-SLC22A2 (OCT2) Antibody, preincubated with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020).

image 2 :

Expression of OCT2 in rat medial septum nucleus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. OCT2 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACT-020

SKU :

ACT-020-CF_0.2 ml

Product Name :

Anti-SLC22A2 (OCT2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O70577

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CT020

Homology :

Mouse - 14 out of 15 amino acid identical; Rat- 12 out of 15 amino acid residues identical It won't recognize human SLC22A2

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(CN)LTADEDTGMKLNPS, corresponding to amino acid residues 321 - 334 of mouse SLC22A2

Product Page - Scientific background :

Organic cation transporters are members of the SLC22A superfamily. These transporters are polyspecific and are involved in the absorption and excretion of various endogenous and exogenous compounds1. The family consists of 18 genes and includes the organic cation transporters (OCTs), organic cation/carnitine transporters (OCTNs), and organic anion transporters (OATs)1-3.Most family members share a common membrane topology consisting of a predicted 12 α-helical transmembrane domains and two hydrophilic loops2,4. Although they share a common structure, the transporters in the SLC22A family show differential tissue distribution. The genes encoding the three organic cation transporters (SLC22A1-3) are all located in a cluster on chromosome 62-4.Organic cation transporter 2 (OCT2) was first identified in the rat in 1996. It is primarily located on the basolateral surface of renal proximal tubules, particularly on the S2 and S3 segments and also expressed in brain.OCT2 mediates the uptake from circulation of several endogenous compounds like creatinine, choline and norepinephrine and also exogenous ones, that include commonly prescribed drugs like the antineoplastic drug cisplatin and the antidiabetic Metformin 5,6.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Organic cation transporter 2, Solute Carrier Family 22 Member 2

Short Description :

A Rabbit Polyclonal antibody to SLC22A2 (OCT2) transporter

Long Description :

Anti-SLC22A2 (OCT2) Antibody (#ACT-020) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC22A2 from rat and mouse samples. The antibody won't recognize human OCT2.

Negative Control :

BLP-CT020

Positive Control :

Synonyms :

Organic cation transporter 2, Solute Carrier Family 22 Member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC22A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage