Anti-SLC22A3 (extracellular) Antibody | Alomone Labs ACT-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC22A3 (extracellular) Antibody

catalog :

ACT-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Otani Y, Yoshikawa S, Nagao K, Tanaka T, Toyooka S, Fujimura A. Connective tissue mast cells store and release noradrenaline. J Physiol Sci. 2023;73:24 pubmed publisher
Matsumoto T, Takayanagi K, Kojima M, Taguchi K, Kobayashi T. Mechanisms underlying suppression of noradrenaline-induced contraction by prolonged treatment with advanced glycation end-products in organ-cultured rat carotid artery. Pflugers Arch. 2020;472:355-366 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200).3,4. Anti-SLC22A3 (extracellular) Antibody, preincubated with SLC22A3 (extracellular) Blocking Peptide (#BLP-CT013).

image 2 :

Expression of OCT3 (SLC22A3) in mouse cortex and hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. OCT3 staining (green) in mouse cortex is detected in neurons (arrows). B. In mouse hippocampus, OCT3 immunoreactivity (green) is observed in astrocytes (vertical arrows) and neurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACT-013

SKU :

ACT-013-CF_0.2 ml

Product Name :

Anti-SLC22A3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88446

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CT013

Homology :

Rat, mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83-96 of rat OCT3

Product Page - Scientific background :

Organic cation transporter 3 (SLC22A3) belongs to the SLC22A gene family. Organic cation transporters (OCTs) are membrane transport proteins involved in many metabolic processes and detoxification. The transporters are responsible for the uptake and intracellular inactivation of numerous endogenous and exogenous substrates including norepinephrine, epinephrine, dopamine, histamine and serotonin. They are also responsible for the uptake of a variety of anticancer drugs1,2. OCT structure is suggested to have 12 transmembrane domains and a large hydrophobic cleft capable of accommodating a wide variety of chemical species3.In humans, OCT3 is expressed in many tissues; abundantly in the placenta, heart, liver and skeletal muscle. It is also expressed in astrocytes, intestinal epithelium neurons, glia, ependymal cells, and blood-cerebrospinal fluid barrier in choroid plexus epithelial cells. The protein is also widely distributed in the brain. OCT expression is detected in several cancer cell lines and tumor tissue samples1-3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

OCT3, Organic cation transporter 3

Short Description :

A Rabbit Polyclonal Antibody to Organic Cation Transporter 3

Long Description :

Anti-SLC22A3 (extracellular) Antibody (#ACT-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Organic cation transporter 3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Negative Control :

BLP-CT013

Positive Control :

Synonyms :

OCT3, Organic cation transporter 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC22A3

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage