Anti-SLC22A1 Antibody | Alomone Labs ACT-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC22A1 Antibody

catalog :

ACT-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Hunter R, Hughey C, Lantier L, Sundelin E, Peggie M, Zeqiraj E, et al. Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase. Nat Med. 2018;24:1395-1406 pubmed publisher

image

image 1 :

Western blot analysis of rat small intestine lysate (lanes 1 and 3) and rat H4-II-E-C3 hepatoma cell lysate (lanes 2 and 4): - 1-2. Anti-SLC22A1 Antibody (#ACT-011), (1:200).3-4. Anti-SLC22A1 Antibody, preincubated with SLC22A1 Blocking Peptide (#BLP-CT011).

image 2 :

Western blot analysis of rat small intestine lysate (lanes 1 and 3) and rat H4-II-E-C3 hepatoma cell lysate (lanes 2 and 4): - 1-2.Anti-SLC22A1 Antibody (#ACT-011) (1:200).3-4. Anti-SLC22A1 Antibody preincubated with the negative control antigen.

image 3 :

Cell surface detection of VPAC1 in live intact mouse J774 macrophage cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-VPAC1 (VIPR1) (extracellular)-FITC Antibody(#AVR-001-F) (5 g).

product information

CAT :

ACT-011

SKU :

ACT-011-CF_0.2 ml

Product Name :

Anti-SLC22A1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63089

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CT011

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ENLGRRKSKAKENT, corresponding to amino acid residues 530- 543 of rat OCT1

Product Page - Scientific background :

Organic cation transporters belong to the Solute carrier family 22 (SLC22) and the MATE family. The SLC22 family contains the three subtypes of passive diffusion organic cation transporters called OCT1 (SLC22A1), OCT2 (SLC22A2) and OCT3 (SLC22A3). OCT1 is responsible for the uptake of organic cationic compounds from blood to hepatocytes1. Like most members of the SLC22 family, the organic cation and carnitine transporters have a predicted membrane topology that comprises 12 α-helical transmembrane domains (TMDs), an intracellular N-terminus, a large glycosylated extracellular loop, a large intracellular loop with phosphorylation sites and an intracellular C-terminus2. In humans, OCT1 has a broad tissue distribution, although it is primarily expressed in hepatocytes3. In rat hepatocytes, OCT1 has been located at the sinusoidal membrane4. OCT1 is also expressed in cholangiocytes5. Human OCT1 is also expressed at the apical membrane of epithelial cells in the proximal and distal tubules of the nephron6. A recent study demonstrated that primary liver cancer derived from epithelial cells, namely, hepatocellular carcinoma (HCC), cholangiocarcinoma (CGC), and hepatoblastoma, shares a decreased expression of OCT1 as a common feature7. The reduction in the expression levels of this transporter has been associated with advanced tumor stages and poorer overall patient survival rates in HCC and CGC5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

OCT1, Organic cation transporter 1

Short Description :

A Rabbit Polyclonal Antibody to Organic Cation Transporter 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Organic Cation Transporter 1. Anti-SLC22A1 Antibody (#ACT-011) can be used in western blot analysis. It has been designed to recognize OCT1 from rat and mouse samples. The antibody is not recommended for human samples.

Negative Control :

BLP-CT011

Positive Control :

Synonyms :

OCT1, Organic cation transporter 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC22A1

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage