Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody | Alomone Labs ACT-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody

catalog :

ACT-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ivanova E, Bianchimano P, Corona C, Eleftheriou C, Sagdullaev B. Optogenetic Stimulation of Cholinergic Amacrine Cells Improves Capillary Blood Flow in Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 2020;61:44 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003), (1:200).3,4. Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody, preincubated with Vesicular Acetylcholine Transporter/VACHT Blocking Peptide (#BLP-CT003).

image 2 :

Expression of VAChT in rat medial septum and spinal cord - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. A. VAChT staining (green) in rat medial septum is detected in neuronal soma (arrows). B. Staining of spinal cord sections reveals VAChT labeling in neuronal soma (arrows). Cell nuclei are stained with DAPI (blue).

image 3 :

Expression of VAChT in rat medial septum and spinal cord - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Vesicular Acetylcholine Transporter(VAChT)Antibody (#ACT-003) (1:400) followed by goat-anti-rabbit-AlexaFluor-488. A. VAChT staining (green) in rat medial septum is detected in neuronal soma (arrows). B. Staining of spinal cord sections reveals VAChT labeling in neuronal soma (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACT-003

SKU :

ACT-003-CF_0.2 ml

Product Name :

Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62666

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CT003

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DAVRLREVQGKDGGE, corresponding to amino acid residues 495-509 of rat VAChT

Product Page - Scientific background :

In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Vesicular acetylcholine transporter (VAChT) transports acetylcholine from the cytoplasm to the lumen of synaptic vesicles where following depolarization, acetylcholine is released in the synaptic cleft and activates muscarinic and nicotinic receptors located on postsynaptic membranes1-3. Acetylcholine released in the synaptic cleft is rapidly hydrolyzed into choline and acetate. Since choline is not de novo synthesized and thereby only made available through diet uptake, choline is recycled back into the cells in order to regenerate acetylcholine1,2.VAChT is a member of the SLC18 family of transporters. It has 12 membrane spanning domains, with extracellular N- and C-terminal tails. The transporter exchanges cytoplasmic acetylcholine for two vesicular protons4-6.VAChT is a selective marker of cholinergic neurons and could be used therefore to determine the loss of cholinergic neurons in Alzheimer's or Alzheimer's related disease7. VAChT knockout mice die after birth. They display a lack of stimulated release of acetylcholine and an underdeveloped neuromuscular junction4.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Solute carrier family 18 member 3, SLC18A3

Short Description :

A Rabbit Polyclonal Antibody to Vesicular Acetylcholine Transporter

Long Description :

Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VAChT from mouse and rat samples. The antibody is not suited to recognize VAChT from human samples.

Negative Control :

BLP-CT003

Positive Control :

Synonyms :

Solute carrier family 18 member 3, SLC18A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC18A3

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage