Anti-Nucleobindin-2/Nesfatin-1 Antibody | Alomone Labs ACS-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nucleobindin-2/Nesfatin-1 Antibody

catalog :

ACS-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat pancreas (lanes 3 and 7) and rat pituitary (lanes 4 and 8): - 1,2,4. Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011), (1:200).3. Anti-Nucleobindin-2/Nesfatin-1 Antibody (1:400).5-8. Anti-Nucleobindin-2/Nesfatin-1 Antibody, preincubated with Nucleobindin-2/Nesfatin-1 Blocking Peptide (#BLP-CS011).

image 2 :

Expression of Nucleobindin-2/Nesfatin-1 in rat hypothalmus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011), (1:1200), followed by goat anti-rabbit-Alexa-488. A. Staining in the ventromedial hypothalamus (VMH) region showed Nucleobindin-2/Nesfatin-1 immunoreactivity (green) in VMH neurons (arrows). B. Pre-incubation of the antibody with Nucleobindin-2/Nesfatin-1 Blocking Peptide (#BLP-CS011), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle

product information

CAT :

ACS-011

SKU :

ACS-011-CF_0.2 ml

Product Name :

Anti-Nucleobindin-2/Nesfatin-1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JI85

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CS011

Homology :

Mouse -13/15 amino acid residues identical; human -11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DKTKVHNVEPVESAR, corresponding to amino acid residues 30-44 of rat Nucleobindin-2/Nesfatin-1

Product Page - Scientific background :

Nucleobindin contains multiple functional domains including a signal peptide on the N-terminal side, a leucine/isoleucine rich region, a DNA binding domain and a putative nuclear targeting signal. The second half of the protein contains two Ca2+-EF-hand motifs and a leucine zipper motif in the C-terminal region. To date, two nucleobindins have been identified, namely nucleobindin 1 and nucleobindin 2.Nucleobindin 2 (NUCB2) can be found on the plasma membrane and in the cytoplasm. Putative post-translational processing of NUCB2 by the enzyme pro-hormone convertase (PC)-1/3 results in nesfatin-1, nesfatin-2 and nesfatin-3. So far, a biological activity has only been demonstrated for nesfatin-1 and its fragment, nesfatin-124-531.The gene Nesfatin/NUCB2 encoding the protein is expressed in the appetite control hypothalamic nuclei such as the paraventricular nucleus (PVN), the supraoptic nucleus, the lateral hypothalamic area and the zona increta in rats. Nefstain-1 immunoreactivity is also detected in the brain stem nuclei such as the nucleus of the solitary tract and the dorsal nucleus of the vagus.Nesfatin-1 has a substantial anorexigenic effect through the selective reduction of nesfatin/NUCB mRNA expression in the PVN of rats under starved conditions. Refeeding of rats was found to restore Nesfatin-1 reduction. A similar effect was not observed in other hypothalamic nuclei2. In humans, Nesfatin-1 levels in the saliva and serum of epilepsy patients are reduced with the use of anti-epileptic medication, but are still higher than the amounts in normal subjects. In addition, nesfatin-1 levels are decreased in human subjects with type-2 diabetes and it has been hypothesized that the observed decrement might be associated with diabetic hyperphagia3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109

Short Description :

A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1

Long Description :

Anti-Nucleobindin-2/Nesfatin-1 Antibody (#ACS-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Nucleobindin-2/Nesfatin-1 from human, mouse, and rat samples.

Negative Control :

BLP-CS011

Positive Control :

Synonyms :

NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NUCB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage