Anti-Calnexin Antibody | Alomone Labs ACS-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Calnexin Antibody

catalog :

ACS-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Gutierrez T, Qi H, Yap M, Tahbaz N, Milburn L, Lucchinetti E, et al. The ER chaperone calnexin controls mitochondrial positioning and respiration. Sci Signal. 2020;13: pubmed publisher
Genova T, Grolez G, Camillo C, Bernardini M, Bokhobza A, Richard E, et al. TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. J Cell Biol. 2017;216:2107-2130 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membrane (lanes 1 and 5), mouse heart membrane (lanes 2 and 6), human breast adenocarcinoma (MCF7) lysate (lanes 3 and 7) and human ovary adenocarcinoma (OVCAR-3) cell line lysate (lanes 4 and 8): - 1-4. Anti-Calnexin Antibody (#ACS-009), (1:400).5-8. Anti-Calnexin Antibody, preincubated with Calnexin Blocking Peptide (#BLP-CS009).

image 2 :

Expression of Calnexin in mouse hippocampus - Immunohistochemical staining of mouse hippocampal CA1 region using Anti-Calnexin Antibody (#ACS-009), (1:200). A. Canx staining (green) appears in neurons (horizontal arrow) and astrocytes (vertical arrow). B. GFAP staining of astrocytic fibers (red). C. Merge of panels A and B demonstrates co-localization in some astrocytes. DAPI is used to stain nuclei (blue).

product information

CAT :

ACS-009

SKU :

ACS-009-CF_0.2 ml

Product Name :

Anti-Calnexin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35565

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CS009

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx

Product Page - Scientific background :

Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a -RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of Shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.Coexpression of calnexin with the voltage-gated potassium channel KV1.2α was found to produce a substantial dose-dependent increase in cell surface KV1.2α channels in transfected mammalian COS-1 cells. In contrast, calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6α subunits3.Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

Short Description :

A Rabbit Polyclonal Antibody to Calnexin

Long Description :

Anti-Calnexin Antibody (#ACS-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples.

Negative Control :

BLP-CS009

Positive Control :

Synonyms :

Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CANX

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400-1:600

more info or order :

Alomone Labs product webpage