Anti-CaBP4 Antibody | Alomone Labs ACS-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaBP4 Antibody

catalog :

ACS-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat eye lysate: - 1. Anti-CaBP4 Antibody (#ACS-004), (1:200).2. Anti-CaBP4 Antibody, preincubated with CaBP4 Blocking Peptide (#BLP-CS004).

image 2 :

Peptide (C)RTYGPLLNRMFGKDR corresponding to amino acid residues 105-120 of mouse CaBP4 (AccessionQ8VHC5). Intracellular.

image 3 :

Western blot analysis of rat placenta lysate (lanes 1 and 4) mouse testes lysate (lanes 2 and 5) and rat skeletal muscle membrane (lanes 3 and 6): - 1-3. Anti-LAT1 (SLC7A5) Antibody(#ANT-105) (1:200).4-6. Anti-LAT1 (SLC7A5) Antibody preincubated with the negative control antigen.

product information

CAT :

ACS-004

SKU :

ACS-004-CF_0.2 ml

Product Name :

Anti-CaBP4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VHC5

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CS004

Homology :

Rat - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RTYGPLLNRMFGKDR, corresponding to amino acid residues 105-120 of mouse CaBP4

Product Page - Scientific background :

Neuronal calcium-binding proteins (CaBP1-8) belong to a subclass of the calmodulin (CaM) superfamily and regulate in particular Ca2+ channel targets in the brain and retina1.CaBP4 contains two globular domains (N- and C-lobe) that each contains a pair of EF- hand motifs connected by a central linker. The second EF-hand in CaBP4 lacks conserved residues in the binding loop and is predicted to not bind Ca2+. CaBP4 interacts structurally with the C-terminal region of CaV1.4 and this Ca2+-dependent interaction has been implicated in the modulation of voltage-dependent CaV1.4 activation2.Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in signal transduction. The CaBP4 isoform regulates voltage-gated Ca2+ channels in retinal photoreceptor cells. In the eye, CaBP4 is localized at the photoreceptor synaptic terminals in rods and cones3. CaBP4 is essential for the development and maintenance of the photoreceptor synapse, likely through functional modulation of the photoreceptor Ca2+ channels, α1F L-type voltage- dependent calcium channels (CaV1.4), and neurotransmitter release4.Mutations in both CaV1.4 and CaBP4 have been identiﬁed in patients suffering from congenital stationary night blindness type 2 (CSNB2), indicating that defects in Ca2+-dependent regulation of CaV1.4 by CaBP4 are closely associated with CSNB2. Indeed, deletion of CaBP4 in the mouse leads to a CSNB2-like phenotype5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Calcium-binding protein 4

Short Description :

A Rabbit Polyclonal Antibody to Calcium-Binding Protein 4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Calcium-binding protein 4. Anti-CaBP4 Antibody (#ACS-004) can be used in western blot analysis. It has been designed to recognize CaBP4 from rat, mouse and human samples.

Negative Control :

BLP-CS004

Positive Control :

Synonyms :

Calcium-binding protein 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CABP4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage