Anti-CaBP2 Antibody | Alomone Labs ACS-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaBP2 Antibody

catalog :

ACS-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of Human ARPE-19 retinal epithelium (lanes 1 and 4), mouse heart (lanes 2 and 5), and rat heart (lanes 3 and 6) lysates: - 1-3. Anti-CaBP2 Antibody (#ACS-003), (1:400).4-6. Anti-CaBP2 Antibody, preincubated with CaBP2 Blocking Peptide (#BLP-CS003).

image 2 :

Peptide (C)KDIGQGDASNLHQK corresponding to amino acid residues 222-235 of rat LAT1 (Accession Q63016). 3rd intracellular loop.

product information

CAT :

ACS-003

SKU :

ACS-003-CF_0.2 ml

Product Name :

Anti-CaBP2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JLK4

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CS003

Homology :

Rat - 12/13 amino acid residues identical; human - 9/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CAKTPWH RGSKER, corresponding to amino acid residues 4-16 of mouse CaBP2

Product Page - Scientific background :

Neuronal Ca2+-binding proteins (CaBP1-5) are a subclass of the calmodulin (CaM) superfamily that regulates specific Ca2+ channel targets in the brain and retina. Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in sensory transduction and disease processes1.CaBP1-5 proteins have four EF hands that form pairs within the N lobe (EF1 and 2) and C lobe (EF3 and 4). The two lobes are structurally independent and connected by a flexible linker. Whereas all four EF hands bind Ca2+ in CaM, EF2 in CaBP1 does not bind Ca2+, and EF1 has reduced selectivity for Ca2+ over Mg2+. EF3 and EF4 in the C lobe of CaBP1 exhibit canonical Ca2+-induced conformational changes2. CaBP-target interactions induce functional changes distinct from those caused by CaM and may diversify neuronal responses to Ca2+ signals. CaBPs interact with and reshape the functional properties of certain voltage-gated Ca2+ channels (CaVs)3.CaBP1 is the best characterized family member and has been shown to regulate inositol 1,4,5-triphosphate receptors (IP3Rs), P/Q-type voltage-gated Ca2+ channels, L-type Ca2+ channels, and the transient receptor potential channel (TRPC5)4. CaBP1 regulates the currents of CaV1 and CaV2 channels in neurons, photoreceptors, and auditory hair cells. CaBP interactions with CaV1 channels may be required for hearing and vision, as mutations that disrupt these interactions cause blindness and deafness5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Calcium-binding protein 2

Short Description :

A Rabbit Polyclonal Antibody to Calcium-Binding Protein 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Calcium-binding protein 2. Anti-CaBP2 Antibody (#ACS-003) can be used in western blot analysis. It has been designed to recognize CaBP2 from rat, mouse and human samples.

Negative Control :

BLP-CS003

Positive Control :

Synonyms :

Calcium-binding protein 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CABP2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage