Anti-CaBP1 Antibody | Alomone Labs ACS-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaBP1 Antibody

catalog :

ACS-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Khakipoor S, Ophoven C, SchrÃ¶dl HÃ¤uÃŸel M, Feuerstein M, Heimrich B, Deitmer J, et al. TGF-Î² signaling directly regulates transcription and functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), via Smad4 in mouse astrocytes. Glia. 2017;65:1361-1375 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-CaBP1 Antibody (#ACS-002), (1:600).3,4. Anti-CaBP1 Antibody, preincubated with CaBP1 Blocking Peptide (#BLP-CS002).

image 2 :

Peptide (C)REAMRKLLGHQVGHR corresponding to amino acid residues 256-270 of rat CaBP1 (AccessionO88751). Intracellular C-terminus.

image 3 :

Expression of Sialin in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections withAnti-SLC17A5 (Sialin) Antibody (#AST-015) (1:200) followed by goat-anti-rabbit-AlexaFluor-488. SLC17A5 immunoreactivity (green) appears in the neuropil of the ventral horn. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACS-002

SKU :

ACS-002-CF_0.2 ml

Product Name :

Anti-CaBP1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88751

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CS002

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REAMRKLLGHQVGHR, corresponding to amino acid residues 256-270 of rat CaBP1

Product Page - Scientific background :

Neuronal Ca2+-binding proteins (CaBP1-5) are a subclass of the calmodulin (CaM) superfamily that regulates specific Ca2+ channel targets in the brain and retina. Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in sensory transduction and disease processes1.CaBP1-5 proteins have four EF hands that form pairs within the N lobe (EF1 and 2) and C lobe (EF3 and 4). The two lobes are structurally independent and connected by a flexible linker. Whereas all four EF hands bind Ca2+ in CaM, EF2 in CaBP1 does not bind Ca2+, and EF1 has reduced selectivity for Ca2+ over Mg2+. EF3 and EF4 in the C lobe of CaBP1 exhibit canonical Ca2+-induced conformational changes2. CaBP-target interactions induce functional changes distinct from those caused by CaM and may diversify neuronal responses to Ca2+ signals. CaBPs interact with and reshape the functional properties of certain voltage-gated Ca2+ channels (CaVs)3.CaBP1 is the best characterized family member and has been shown to regulate inositol 1,4,5-triphosphate receptors (IP3Rs), P/Q-type voltage-gated Ca2+ channels, L-type Ca2+ channels, and the transient receptor potential channel (TRPC5)4. CaBP1 regulates the currents of CaV1 and CaV2 channels in neurons, photoreceptors, and auditory hair cells. CaBP interactions with CaV1 channels may be required for hearing and vision, as mutations that disrupt these interactions cause blindness and deafness5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Calcium-binding protein 1, Caldendrin, Calbrain

Short Description :

A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Calcium-binding protein 1. Anti-CaBP1 Antibody (#ACS-002) can be used in western blot analysis. It has been designed to recognize CaBP1 from rat, mouse and human samples.

Negative Control :

BLP-CS002

Positive Control :

Synonyms :

Calcium-binding protein 1, Caldendrin, Calbrain

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CABP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage