Anti-NCS1 Antibody | Alomone Labs ACS-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NCS1 Antibody

catalog :

ACS-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat eye (lanes 3 and 6) lysates (1:200-1:400): - 1-3. Anti-NCS1 Antibody (#ACS-001), (brain lysates 1:200, eye lysate 1:400).4-6. Anti-NCS1 Antibody, preincubated with NCS1 Blocking Peptide (#BLP-CS001).

image 2 :

Expression of NCS1 in mouse cerebellum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NCS1 Antibody (#ACS-001), (1:100), followed by goat anti-rabbit-Alexa-488. A. NCS1 immunoreactivity (green) appeared in the Purkinje layer (P, horizontal arrow) and in purkinje dendrites (vertical arrow) in the molecular layer (MOL). B. Pre-incubation of the antibody with NCS1 Blocking Peptide (BLP-CS001), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACS-001

SKU :

ACS-001-CF_0.2 ml

Product Name :

Anti-NCS1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P62168

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CS001

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1

Product Page - Scientific background :

Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons. The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP

Short Description :

A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Neuronal calcium sensor 1. Anti-NCS1 Antibody (#ACS-001) can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse and human samples.

Negative Control :

BLP-CS001

Positive Control :

Synonyms :

Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NCS1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage