Anti-GPR55 (extracellular) Antibody | Alomone Labs ACR-062 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR55 (extracellular) Antibody

catalog :

ACR-062

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3), and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-GPR55 (extracellular) Antibody (#ACR-062), (1:500). 3, 4. Anti-GPR55 (extracellular) Antibody, preincubated with GPR55 (extracellular) Blocking Peptide (#BLP-CR062).

image 2 :

Expression of GPR55 in rat substantia nigra. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR55 (extracellular) Antibody (#ACR-062), followed by goat anti-rabbit-AlexaFluor-488. A. GPR55 immunoreactivity (green) appears in the substantia nigra pars compacta (SNC) in neurons (arrows). B. Pre-incubation of the antibody with GPR55 (extracellular) Blocking Peptide (BLP-CR062), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.

product information

CAT :

ACR-062

SKU :

ACR-062-CF_0.2 ml

Product Name :

Anti-GPR55 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q3UJF0

Applications :

IFC IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR062

Homology :

Mouse - 14/15 amino acid residues identical; rat - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55

Product Page - Scientific background :

G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo-, phyto- and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.GPR55 is only 13.5% identical to the canonic cannabinoid receptor CB1 and 14.4% identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.GPR55 has a role in self-renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 55

Short Description :

A Rabbit Polyclonal Antibody to GPR55

Long Description :

Anti-GPR55 (extracellular) Antibody (#ACR-062) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the human protein.

Negative Control :

BLP-CR062

Positive Control :

Synonyms :

G-Protein Coupled Receptor 55

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR55

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage