Anti-GPR55 (extracellular)-FITC Antibody | Alomone Labs ACR-062-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR55 (extracellular)-FITC Antibody

catalog :

ACR-062-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of GPR55 in live intact mouse J774 macrophage cells: Cell surface detection of GPR55 in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-GPR55 (extracellular)-FITC Antibody (#ACR-062-F), (2.5 µg).

product information

CAT :

ACR-062-F

SKU :

ACR-062-F_50 mcl

Product Name :

Anti-GPR55 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q3UJF0

Applications :

FC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR062

Homology :

Mouse - 14/15 amino acid residues identical; Rat - 11/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55

Product Page - Scientific background :

G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo-, phyto- and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.GPR55 is only 13.5% identical to the canonic cannabinoid receptor CB1 and 14.4% identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.GPR55 has a role in self-renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 55

Short Description :

A Rabbit Polyclonal Antibody to GPR55 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-GPR55 (extracellular) Antibody (#ACR-062) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the protein from human samples. Anti-GPR55 (extracellular)-FITC Antibody (#ACR-062-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry. The antibody will not recognize the protein from human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

G-Protein Coupled Receptor 55

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR55

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACR-062), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage