Anti-Human GPR55 (extracellular) Antibody | Alomone Labs ACR-061 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human GPR55 (extracellular) Antibody

catalog :

ACR-061

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human NK-92MI natural killer cell (lanes 1 and 3) (1:500) and human acute monocytic leukemia THP-1 cell (lanes 2 and 4) (1:200) lysates: - 1, 2. Anti-Human GPR55 (extracellular) Antibody (#ACR-061). 3, 4. Anti-Human GPR55 (extracellular) Antibody, preincubated with Human GPR55 (extracellular) Blocking Peptide (#BLP-CR061) (#BLP-CR061).

product information

CAT :

ACR-061

SKU :

ACR-061-CF_0.2 ml

Product Name :

Anti-Human GPR55 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y2T6

Applications :

IFC WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CR061

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Peptide (C)HGKVEKYMSFHNMSD, corresponding to amino acid residues 160-174 of human GPR55

Product Page - Scientific background :

GPR55 is an endocannabinoid receptor that belongs to group δ of the rhodopsin-like (class A) receptors. Like all other G-protein coupled receptors, it consists of seven trans-membrane helixes, intracellular C-terminal domain and extracellular N-terminal extension1.The other known cannabinoid receptors, CB1 and CB2 share low homology with GPR55 and have different ligand specificity and different downstream activated cascade. Thus, GPR55 consists on its own a class of cannabinoid receptor2.GPR55 is highly expressed in the striatum and binds a wide range of ligands, among which are endocannabinoids: Anadanine and 2-Arachidonoyl Glycerol, and phytocannabinoids: Tetrahydrocannabinol (THC), abnormal cannabinoid (CBD) and cannabidiol3. Following ligand binding, GPR55 couples to Gα12,13 proteins, which eventually lead to increased intracellular Ca2+ levels2.The endocannabinoid system has a tremendous potential for experimental and clinical research, based on their evolutionary conservation and role in fundamental physiological and pathological processes, including pain modulation, immune function, neuroprotection, cancer, cardiovascular diseases, fertility and appetite4. Clinical studies using cannabinoid receptor agonists and antagonists have suggested their possible use in modulating acute and/or chronic diseases such as hypertension, glaucoma, emesis, anxiety, depression, gastrointestinal and hepatic disorders, ulcerative colitis and neuropathic and inflammatory pain5.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 55

Short Description :

A Rabbit Polyclonal Antibody to GPR55

Long Description :

Anti-Human GPR55 (extracellular) Antibody (#ACR-061) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human samples only.

Negative Control :

BLP-CR061

Positive Control :

Synonyms :

G-protein coupled receptor 55

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR55

AB Specifictiy :

The antibody will not recognize the protein from rat or mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:1000

more info or order :

Alomone Labs product webpage