Anti-CRLR/CALCRL (extracellular) Antibody | Alomone Labs ACR-060 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CRLR/CALCRL (extracellular) Antibody

catalog :

ACR-060

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and rat heart membranes (lanes 3 and 6): - 1-3. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 4-6. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).

image 2 :

Expression of Calcitonin Receptor-Like Receptor in rat brain stem - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in neurons (arrows). B. Pre-incubation of the antibody with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACR-060

SKU :

ACR-060-CF_0.2 ml

Product Name :

Anti-CRLR/CALCRL (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q16602

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR060

Homology :

Mouse, rat - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor

Product Page - Scientific background :

Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.CGRP receptor is a heterodimer composed of two proteins: a 7-transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR

Short Description :

A Rabbit Polyclonal Antibody to Calcitonin Receptor-Like Receptor

Long Description :

Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. It has been designed to recognize CRLR from human, rat, and mouse samples.

Negative Control :

BLP-CR060

Positive Control :

Synonyms :

Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CALCRL

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage