Anti-Human CX3CR1 (extracellular) Antibody | Alomone Labs ACR-059 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CX3CR1 (extracellular) Antibody

catalog :

ACR-059

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human NK-92MI natural killer cells (lanes 1 and 3) and human THP-1 acute monocytic leukemia (lanes 2 and 4) cell lysates: - 1,2. Anti-Human CX3CR1 (extracellular) Antibody (1:600), (#ACR-059).3,4. Anti-Human CX3CR1 (extracellular) Antibody, preincubated with Human CX3CR1 (extracellular) Blocking Peptide (#BLP-CR059).

image 2 :

Cell surface detection of CX3CR1 in human THP-1 monocytic leukemia cells: - ___ Cells.___ Cells + FITC-conjugated goat anti-rabbit antibody. ___ Cells + Anti-Human CX3CR1 (extracellular) Antibody (#ACR-059) (2.5 g/5x105 cells) +FITC-conjugated goat anti-rabbit antibody.

product information

CAT :

ACR-059

SKU :

ACR-059-CF_0.2 ml

Product Name :

Anti-Human CX3CR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49238

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CR059

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)ESVTENFEYDDLAE, corresponding to amino acid residues 6-19 of human CX3CR1

Product Page - Scientific background :

CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Short Description :

A Rabbit Polyclonal Antibody to CX3CR1 Chemokine Receptor

Long Description :

Anti-Human CX3CR1 (extracellular) Antibody (#ACR-059) is a highly specific antibody directed against an epitope of human CX3C chemokine receptor 1. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CX3CR1 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

Negative Control :

BLP-CR059

Positive Control :

Synonyms :

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CX3CL1

AB Specifictiy :

The antibody will not recognize the protein from rat or mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage