Anti-CX3CR1 (extracellular) Antibody | Alomone Labs ACR-058 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CX3CR1 (extracellular) Antibody

catalog :

ACR-058

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen lysate (lanes 3 and 6): - 1-3. Anti-CX3CR1 (extracellular) Antibody (#ACR-058), (1:200). 4-6. Anti-CX3CR1 (extracellular) Antibody, preincubated with CX3CR1 (extracellular) Blocking Peptide (#BLP-CR058).

image 2 :

Expression of CX3CR1 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CX3CR1 (extracellular) Antibody (#ACR-058), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CX3CR1 staining (green) in the hippocampal CA1 region is detected in microglial profiles in the stratum oriens. Some profiles include the entire microglial outline including soma (horizontal arrows) while other outlines are processes (vertical arrows). B. Pre-incubation of the antibody with CX3CR1 (extracellular) Blocking Peptide (#BLP-CR058), suppresses staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SR = stratum radiatum, SO = stratum oriens.

product information

CAT :

ACR-058

SKU :

ACR-058-CF_0.2 ml

Product Name :

Anti-CX3CR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0D9

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR058

Homology :

Rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1

Product Page - Scientific background :

CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's Disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Short Description :

A Rabbit Polyclonal Antibody to CX3CR1 Receptor

Long Description :

Anti-CX3CR1 (extracellular) Antibody (#ACR-058) is a highly specific antibody directed against an epitope of the mouse receptor. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize the protein from human samples.

Negative Control :

BLP-CR058

Positive Control :

Synonyms :

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CX3CR1

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage