Anti-CX3CR1 (extracellular)-APC Antibody | Alomone Labs ACR-058-APC product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CX3CR1 (extracellular)-APC Antibody

catalog :

ACR-058-APC

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse BV-2 microglia cells:

image 2 :

Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse J774 macrophage cells:

product information

CAT :

ACR-058-APC

SKU :

ACR-058-APC_50 mcl

Product Name :

Anti-CX3CR1 (extracellular)-APC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0D9

Applications :

FC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR058

Homology :

Rat - identical Will not recognize human CX3CR1

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus.

Label :

Allophycocyanin (APC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1

Product Page - Scientific background :

CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's Disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Short Description :

A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

Long Description :

Anti-CX3CR1 (extracellular) Antibody (#ACR-058) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize human CX3CR1. Anti-CX3CR1 (extracellular)-APC Antibody (#ACR-058-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize human CX3CR1.

Negative Control :

RIC-001-APC

Positive Control :

Synonyms :

Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

Cx3cr1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACR-058), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage