Anti-Calcitonin Receptor (extracellular) Antibody | Alomone Labs ACR-053 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Calcitonin Receptor (extracellular) Antibody

catalog :

ACR-053

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Long Q, Wang H, Gao W, Fan Y, Li Y, Ma Y, et al. Long Noncoding RNA Kcna2 Antisense RNA Contributes to Ventricular Arrhythmias via Silencing Kcna2 in Rats With Congestive Heart Failure. J Am Heart Assoc. 2017;6: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-Calcitonin Receptor (extracellular) Antibody (#ACR-053), (1:200).3,4. Anti-Calcitonin Receptor (extracellular) Antibody, preincubated with Calcitonin Receptor (extracellular) Blocking Peptide (#BLP-CR053).

image 2 :

Expression of CALCR in rat PC12 cells - Cell surface detection ofCALCR in intact living rat pheochromocytoma (PC12) cells. A C. Extracellular staining of cells usingAnti-Calcitonin Receptor (extracellular)Antibody (#ACR-053) (1:50). B D. Merged images of A and C each with its respective live view of the cells.

product information

CAT :

ACR-053

SKU :

ACR-053-CF_0.2 ml

Product Name :

Anti-Calcitonin Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32214

Applications :

IC IF LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR053

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HLVEVVPNGDLVRRD, corresponding to amino acid residues 201-215 of rat CALCR

Product Page - Scientific background :

the calcitonin receptor (CTR) is a class B G-protein coupled receptor (GPCR) that signals via activation of cAMP and protein kinase C (PKC). It is one of 15 human class B GPCRs1,2. Apart from CTR, calcitonin receptor-like receptor (CTLR) is the only other B-class GPCR to bind calcitonin. To date, there are three known human CTR mRNA isoforms arising from alternative splicing3.Calcitonin itself is a peptide hormone that contributes to bone calcification by direct inhibition of osteoclasts which resorb the bony tissue. It is part of a six-member family that has varied functionalities. By binding to receptor activity modifying proteins (RAMPs), CTR and CTLR can form receptors to every member of the calcitonin family. For instance, RAMP1 and CTR construct the calcitonin gene-related peptide (CGRP) receptor.Both CTR and CTLR have seven transmembrane domains, an intracellular C-terminal, and an extracellular N-terminal. To activate the receptors, their N-terminal must first capture the C-terminal of the ligand and then carried to the transmembrane loops where it bestows a conformational change upon them.Besides the bone, CTR has prominent roles in the kidney, CNS, placenta, ovaries, sperm, lung, and in the brain4,5.In osteoclasts, the expression of CTRs declines with age. Interestingly, polymorphism of the CTR gene is strongly associated with decreased fracture-risk in post-menopausal women3,6.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

CALCR, CT receptor, CT-R, C1A/C1B

Short Description :

A Rabbit Polyclonal Antibody to Calcitonin Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CT-R. Anti-Calcitonin Receptor Antibody (#ACR-053) can be used in western blot and live cell imaging applications. It has been designed to recognize CT-R from human, rat and mouse samples.

Negative Control :

BLP-CR053

Positive Control :

Synonyms :

CALCR, CT receptor, CT-R, C1A/C1B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CALCR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage