Anti-CNTFRα (extracellular) Antibody | Alomone Labs ACR-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CNTFRα (extracellular) Antibody

catalog :

ACR-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-CNTFRα (extracellular) Antibody (#ACR-051), (1:200).4-6. Anti-CNTFRα (extracellular) Antibody, preincubated with CNTFRα (extracellular) Blocking Peptide (#BLP-CR051).

image 2 :

Expression of CNTF receptor α in rat hippocampus - Immunohistochemical staining of rat hippocampal CA1 region using Anti-CNTFRα (extracellular) Antibody (#ACR-051), (1:400). A. CNTFRα staining (green) appears in astrocytes in the CA1 region (arrows). B. Nuclei staining using DAPI as the counterstain (green). C. Merged image of panels A and B.

image 3 :

Expression of CNTF receptor ? in rat hippocampus - Immunohistochemical staining of rat hippocampal CA1 region usingAnti-CNTFR? (extracellular)Antibody (#ACR-051) (1:400). A. CNTFR? staining (green) appears in astrocytes in the CA1 region (arrows). B. Nuclei staining using DAPI as the counterstain (green). C. Merged image of panels A and B.

product information

CAT :

ACR-051

SKU :

ACR-051-CF_0.2 ml

Product Name :

Anti-CNTFRα (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q08406

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR051

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CHIRYMHLFSTIKYK, corresponding to amino acid residues 164-178 of rat CNTFRα

Product Page - Scientific background :

CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFRα)5.CNTFRα is anchored to the membrane by a glycosyl-phosphatidylinositol (GPI) linkage and lacks the intracellular signaling moiety. It can be cleaved from the GPI anchor to yield a soluble form of the receptor (sCNTFRα)6,7.CNTF binds to CNTFRα, the latter associates with gp130 which recruits LIFR. This leads to the activation of a signaling cascade involving the JAK/STAT pathway7.CNTFRα knockout mice die within 24 hours after birth as opposed to CNTF knock animals which are viable. This strongly suggest that CNTFRα has other ligands besides CNTF. Indeed, CLC/CLF complex (cardiotrophin like cytokine/cytokine-like factor-1) binds CNTFRα and activates a similar signaling pathway to that of CNTF8.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha

Short Description :

A Rabbit Polyclonal Antibody to CNTF Receptor α

Long Description :

Anti-CNTFRα (extracellular) Antibody (#ACR-051) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize CNTF receptor α from human, mouse, and rat samples.

Negative Control :

BLP-CR051

Positive Control :

Synonyms :

Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNTFR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage