Anti-CRF1/CRHR1 (extracellular) Antibody | Alomone Labs ACR-050 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CRF1/CRHR1 (extracellular) Antibody

catalog :

ACR-050

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6): - 1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050).

image 2 :

Expression of Corticotropin-releasing factor receptor 1 in mouse brain - Immunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:600). A. CRF1 staining (green) in the cerebellum appears in cerebellar molecular layer (MOL). B. CRF1 staining (green) in the hippocampus appears in soma (horizontal arrows) and dendrites of pyramidal neurons (arrow). In both panels DAPI was used as the counterstain.

product information

CAT :

ACR-050

SKU :

ACR-050-CF_0.2 ml

Product Name :

Anti-CRF1/CRHR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35353

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR050

Homology :

Mouse -12/14 amino acid residues identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1

Product Page - Scientific background :

Corticotrophin-releasing factor (CRF) is a peptide hormone and a key regulator of the stress response. Through activation of the hypothalamic-pituitary axis and its role as a neurotransmitter, CRF influences a wide range of physiological responses, including appetite control, cardiovascular regulation, glucose metabolism, immune function and behavior.Corticotrophin-releasing factor receptor 1 (CRHR1, CRF1) is a member of the class B G-protein coupled receptors. CRHR1 is activated by CRF. It is expressed in brain areas including the hypothalamus, pituitary, amygdala and cortex. The core fold of the receptor features seven transmembrane helices (TM1-TM7). The connecting loops lack secondary structure apart from one extracellular loop- ECL1. In contrary to A Class GPCRs, CRF1 has a pronounced V-shape and a large cavity hypothesized to be the peptide binding site. The cytoplasmic half of TM3 and TM5 interacts with Gs. Small molecule antagonists of the receptor, such as CP-376395, act allosterically by maintaining the receptor in its inactive state at a site different from the peptide binding site. Interestingly, although CP-376395 is highly selective for CRHR1, all of the residues found to interact directly with this ligand are conserved between CRHR1 and CRHR21.Interaction between childhood trauma and sequence variation in the CRHR1 gene that increase risk for affective disorders has been established by several studies. In Rhesus macaques, single nucleotide polymorphisms affecting exon 6 of CRHR1 gene influence anxious temperament (a phenotype that predicts the development of human anxiety and depressive disorder) and brain metabolism2.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1

Short Description :

A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1

Long Description :

Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050) is a highly specific antibody directed against an extracellular epitope of the rat Corticotropin-releasing factor receptor 1. It can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize CRFR1 from human, mouse, and rat samples.

Negative Control :

BLP-CR050

Positive Control :

Synonyms :

Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CRHR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage