Anti-CCKBR (extracellular) Antibody | Alomone Labs ACR-042 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CCKBR (extracellular) Antibody

catalog :

ACR-042

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200).3,4. Anti-CCKBR (extracellular) Antibody, preincubated with CCKBR (extracellular) Blocking Peptide (#BLP-CR042).

image 2 :

Expression of Cholecystokinin B receptor in rat stomach - Immunohistochemical staining of paraffin embedded rat stomach sections using Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:100). Cholecystokinin B receptor (brown) is expressed in both parietal cells (black arrows) and in chief cells (red arrows) of the gastric mucosa. Hematoxilin is used as the counterstain.

image 3 :

Cell surface detection of Cholecystokinin B receptor in live intact Jurkat (human T cell leukemia) cell lines: - ___Unstained cells + goat-anti-rabbit-Phycoerythrin (PE).___Cells + Anti-CCKBR (extracellular) Antibody (#ACR-042) (1:20) + goat-anti-rabbit-PE.

product information

CAT :

ACR-042

SKU :

ACR-042-CF_0.2 ml

Product Name :

Anti-CCKBR (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56481

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR042

Homology :

Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CETPRIRGTGTRELE, corresponding to amino acid residues 39-53 of mouse Gastrin/cholecystokinin type B receptor

Product Page - Scientific background :

Cholecystokinin B receptor (CCKBR), also known as the Gastrin receptor and CCKR2, belongs to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily, and is one of the two receptors that mediates the effects of the cholecystokinin (CCK) and gastrin peptides1,2.CCK and gastrin are related linear peptides that occur in different forms but share the last five C-terminal amino acid residues. CCK regulates several nutritional-related activities such as stimulation of pancreatic exocrine secretion or the regulation of intestinal transit, while gastrin's main function is the stimulation of gastric acid secretion1,2.Both CCKBR and the other CCK receptor (CCKAR or CCK1R) are coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), and intracellular Ca2+mobilization.CCKBR was originally identified in the brain (and hence its name, type B for Brain) where it is widely distributed, notably in the cerebral cortex and striatum. In the periphery, CCKBR is most notably expressed in acid secreting cells in the mucosa of the stomach. The tissue distribution of CCKBR corresponds to the proposed roles of the receptor which include anxiety, pain perception, gastric acid secretion, and growth and differentiation of the gastric mucosa1,2.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Cholecystokinin B receptor, Gastrin receptor, CCK2R, GASR

Short Description :

A Rabbit Polyclonal Antibody to the Cholecystokinin B Receptor

Long Description :

Anti-CCKBR (extracellular) Antibody (#ACR-042) is a highly specific antibody directed against an epitope of the mouse cholecystokinin B receptor. The antibody can be used in western blot, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize CCKBR from mouse, rat, and human samples.

Negative Control :

BLP-CR042

Positive Control :

Synonyms :

Cholecystokinin B receptor, Gastrin receptor, CCK2R, GASR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CCKBR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage