Anti-Rat CMKLR1 (extracellular) Antibody | Alomone Labs ACR-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Rat CMKLR1 (extracellular) Antibody

catalog :

ACR-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Ikarashi N, Mizukami N, Pei C, Uchino R, Fujisawa I, Fukuda N, et al. Role of Cutaneous Aquaporins in the Development of Xeroderma in Type 2 Diabetes. Biomedicines. 2021;9: pubmed publisher
Joya A, Ardaya M, Montilla A, Garbizu M, Plaza Garc xed a S, G xf3 mez Vallejo V, et al. In vivo multimodal imaging of adenosine A1 receptors in neuroinflammation after experimental stroke. Theranostics. 2021;11:410-425 pubmed publisher
Ikarashi N, Kon R, Nagoya C, Ishikura A, Sugiyama Y, Takahashi J, et al. Effect of Astaxanthin on the Expression and Activity of Aquaporin-3 in Skin in an In-Vitro Study. Life (Basel). 2020;10: pubmed publisher
Ikarashi N, Kaneko M, Watanabe T, Kon R, Yoshino M, Yokoyama T, et al. Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Erlotinib Induces Dry Skin via Decreased in Aquaporin-3 Expression. Biomolecules. 2020;10: pubmed publisher
Papanikolaou M, Lewis A, Butt A. Glial and neuronal expression of the Inward Rectifying Potassium Channel Kir7.1 in the adult mouse brain. J Anat. 2019;235:984-996 pubmed publisher
Ikarashi N, Nagoya C, Kon R, Kitaoka S, Kajiwara S, Saito M, et al. Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption. Int J Mol Sci. 2019;20: pubmed publisher

image

image 1 :

Western blot analysis of rat cerebellum lysate (lanes 1 and 3) and rat heart membrane (lanes 2 and 4): - 1,2. Anti-Rat CMKLR1 (extracellular) antibody (1:200), (ACR-033).3,4. Anti-Rat CMKLR1 (extracellular) antibody, preincubated with Rat CMKLR1 (extracellular) Blocking Peptide (#BLP-CR033).

product information

CAT :

ACR-033

SKU :

ACR-033-CF_0.2 ml

Product Name :

Anti-Rat CMKLR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35786

Applications :

IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR033

Homology :

Rat only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HSTHPRTDPVGYSRH, corresponding to amino acid residues 201-215 of rat Chemerin receptor 1

Product Page - Scientific background :

Chemerin Receptor 1 belongs to class A G-protein coupled receptors. The receptor is coupled to Gi/o, leading to an increase in intracellular cAMP and Ca2+ following activation by the binding of Chemerin, its endogenous ligand. Like all GPCRs, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.Chemerin Receptor 1 has numerous names depending on how the gene encoding the receptor was cloned: Chemokine-like receptor 1, CMKLR1, ChemR23 and others all correspond to the same gene/protein1.Expression of Chemerin receptor 1 at the mRNA level is largely detected in adipose tissue, and skin dermal layer. At the protein level, CMKLR1 is expressed at the cell surface of dendritic cells, monocytes and macrophages, smooth muscle cells, adipose tissue, lung, lymph nodes and testis1.A role for the receptor in the inflammatory response has been attributed, however data indicate contradictory roles; proinflammatory and anti-inflammatory functions of the receptor. Expression of Chemerin receptor 1 in adipose tissue clearly establishes its involvement in energy metabolism as an important regulator of adipogenesis1.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ

Short Description :

A Rabbit Polyclonal Antibody to Chemerin Receptor 1

Long Description :

Anti-Rat CMKLR1 (extracellular) Antibody (#ACR-033) is a highly specific antibody directed against an epitope of rat Chemerin receptor 1. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize the protein from rat samples only. The antibody is unlikely to recognize the receptor from human and mouse samples.

Negative Control :

BLP-CR033

Positive Control :

Synonyms :

Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CMKLR1

AB Specifictiy :

Will unlikely recognize CMKLR1 from human and mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage