Anti-Human CCR5 (extracellular) Antibody | Alomone Labs ACR-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CCR5 (extracellular) Antibody

catalog :

ACR-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 4), human THP-1 acute monocytic leukemia (lanes 2 and 5) and human MEG-O1 Chronic myelogenous leukemia (lanes 3 and 6) cell lysates: - 1-3. Anti-Human CCR5 (extracellular) Antibody (#ACR-025), (1:400).4-6. Anti-Human CCR5 (extracellular) Antibody, preincubated with Human CCR5 (extracellular) Blocking Peptide (#BLP-CR025).

product information

CAT :

ACR-025

SKU :

ACR-025-CF_0.2 ml

Product Name :

Anti-Human CCR5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51681

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CR025

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CSSHFPYSQYQFWKN, corresponding to amino acid residues 178-192 of human CCR5

Product Page - Scientific background :

CCR5 protein is a G-protein coupled receptor (GPCR) responsible for regulating host immune responses and functions against pathogens. The protein plays a key role in transferring immune cells to inflammatory sites and injury. CCR5 is also considered to be an anchor protein for R5-tropic strains of HIV-1 (Human Immunodeficiency Virus-1) to infect immune cells. Thus, CCR5 contributes to the development of AIDS (Acquired Immunodeficiency Syndrome).The receptor is widely expressed in a variety of immune cells involved in the inflammatory responses such as macrophages, monocytes, T cells and dendritic cells1,2.CCR5 is the receptor for the CC ligand 3 (CCL3), CC ligand 4 (CCL4), and CC ligand 5 (CCL5) chemokine ligands. CCR5 and its ligands are upregulated during HIV infection.Studies have shown that lack of homozygosity of CCR5Δ32 variant, a 32 base-pair deletion of the CCR5's gene coding region, is associated with preventing the risk of HIV-1 infection2,3.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195

Short Description :

A Rabbit Polyclonal Antibody to CCR5 Chemokine Receptor

Long Description :

Anti-Human CCR5 (extracellular) Antibody (#ACR-025) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize CCR5 from human samples only. The antibody is unlikely to recognize the receptor from mouse and rat samples.

Negative Control :

BLP-CR025

Positive Control :

Synonyms :

C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CCR5

AB Specifictiy :

Not recommended for use with rat and mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage