Anti-Human CXCR5 (extracellular) Antibody | Alomone Labs ACR-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CXCR5 (extracellular) Antibody

catalog :

ACR-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of Burkitt's lymphoma (Raji) (lanes 1 and 4), human prostate carcinoma (LNCaP) (lanes 2 and 5) and human prostate carcinoma (PC-3) cell line lysates (lanes 3 and 6): - 1-3. Anti-Human CXCR5 (extracellular) Antibody (#ACR-015), (1:400).4-6. Anti-Human CXCR5 (extracellular) Antibody, preincubated with Human CXCR5 (extracellular) Blocking Peptide (#BLP-CR015).

product information

CAT :

ACR-015

SKU :

ACR-015-CF_0.2 ml

Product Name :

Anti-Human CXCR5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32302

Applications :

IC IF IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CR015

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

RLDNYNDTSLVENHLC, corresponding to amino acid residues 23-38 of human CXCR5

Product Page - Scientific background :

Chemokine receptors are a family of receptors that signal upon binding of one or more members of the chemokine superfamily of chemotactic cytokines. Chemokines coordinate development, differentiation and trafficking of various immune system cells making them key players of the innate and adaptive immune response.CXCR5 (also known as Burkitt lymphoma receptor 1) belongs to the CXC subfamily of chemokine receptors and is a G-coupled protein receptor with 7 transmembrane segments. There is considerable homology between CXCR5 and other members of the chemokine receptor family such as IL-9 receptor, CCR-1 and BLR2. CXCR5 expression is detected in peripheral blood CD4 and CD8 leukocytes as well as in secondary lymphatic organs and in granule and Purkinje layers in the brain. It is also expressed in Burkitt's lymphoma cells1.CXCR5 is activated by B-cell attracting chemokine 1 (BCA-1) that is chemotactic for human B lymphocytes. BCA-1 does not affect IL-2 stimulated T-cells, monocytes or neutrophils2. CXCR5 mediates correct T cell localization within granulomas, efficient macrophage activation and lymphoid follicle formation3.CXCR5 knockout mice have a severe defect in normal B cell migration and localization. Knockout mice lack inguinal lymph nodes, have a reduced amount of Peyer's patches and have abnormal primary lymphoid follicles and no functional germinal centers in the spleen4. Absence of CXCR5 has been implicated in increased susceptibility to tuberculosis to due defective T cell localization within the lung3.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, BLR1, Monocyte-derived receptor 15, MDR15, CD185

Short Description :

A Rabbit Polyclonal Antibody to CXCR5 Chemokine Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human CXCR5. Anti-Human CXCR5 (extracellular) Antibody (#ACR-015) can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR5 from human samples only.

Negative Control :

BLP-CR015

Positive Control :

Synonyms :

C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, BLR1, Monocyte-derived receptor 15, MDR15, CD185

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CXCR5

AB Specifictiy :

Will not recognize rat and mouse CXCR5.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage