Anti-CXCR4 (extracellular) Antibody | Alomone Labs ACR-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CXCR4 (extracellular) Antibody

catalog :

ACR-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Albee L, Gao X, Majetschak M. Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells. PLoS ONE. 2021;16:e0253821 pubmed publisher
Chen C, Chen Y, Li M, Xiao H, Shi Q, Zhang T, et al. Functional decellularized fibrocartilaginous matrix graft for rotator cuff enthesis regeneration: A novel technique to avoid in-vitro loading of cells. Biomaterials. 2020;250:119996 pubmed publisher
Ciullo A, Biemmi V, Milano G, Bolis S, Cervio E, Fertig E, et al. Exosomal Expression of CXCR4 Targets Cardioprotective Vesicles to Myocardial Infarction and Improves Outcome after Systemic Administration. Int J Mol Sci. 2019;20: pubmed publisher
Gao X, Abdelkarim H, Albee L, Volkman B, Gaponenko V, Majetschak M. Partial agonist activity of α1-adrenergic receptor antagonists for chemokine (C-X-C motif) receptor 4 and atypical chemokine receptor 3. PLoS ONE. 2018;13:e0204041 pubmed publisher
Gao X, Albee L, Volkman B, Gaponenko V, Majetschak M. Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by Î±1-adrenergic receptors at the heteromeric receptor complex. Sci Rep. 2018;8:2730 pubmed publisher
Albee L, Eby J, Tripathi A, Laporte H, Gao X, Volkman B, et al. ?1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc. 2017;6: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:200).3,4. Anti-CXCR4 (extracellular) Antibody, preincubated with CXCR4 (extracellular) Blocking Peptide (#BLP-CR014).

image 2 :

Expression of CXCR4 in rat cerebellum - Immunohistochemical staining of rat cerebellum frozen section using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:100). CXCR4 is expressed in Purkinje cell bodies and axonal prolongations (arrows). Hoechst 33342 is used as the counterstain.

image 3 :

Cell surface detection of CXCR4 in Jurkat living cells: - ___Unstained cells.___Cells +Anti-CXCR4 (extracellular)Antibody (#ACR-014) (10 g/0.5x106cells).

product information

CAT :

ACR-014

SKU :

ACR-014-CF_0.2 ml

Product Name :

Anti-CXCR4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P61073

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CR014

Homology :

Mouse - 12/14 amino acid residues identical; rat - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4

Product Page - Scientific background :

Chemokines are small molecular weight, soluble secreted proteins that bind and activate their respective G-protein coupled receptor (GPCR), chemokine receptors in order to evoke a cellular response resulting in migration or chemotaxis1.The chemokine system involves more than 40 chemokines and 18 chemokine receptors. The receptors are designated CXCR1-5, CCR1-11, XCR1 and CX3CR1, based on their specific ligand preference2.Chemokine receptors are present on many different cell types. They were initially detected on leukocytes, where they were found to play an important role in the migration of these cells to inflammation sites3.CXCR4 was originally identified as an orphan receptor, and soon gained much attention when it was discovered as a coreceptor for HIV-14. Besides from being involved in HIV-1 infection/progression, CXCR4 is found to be upregulated in many different cancers/tumors and has evolved to become a target for the development of antagonists5. CXCL12 (SDF-1α) is the sole ligand for CXCR4. Following binding of its ligand, CXCR4 undergoes dimerization and activates Gi G-proteins6,7. However downstream activation through CXCR4 could also occur through other G-proteins and non-G-proteins5. The down regulation of the CXCR4 receptor is initiated by phosphorylation of its cytoplasmic tail, which is followed by the binding of arrestin. The receptor is then internalized through endocytosis and degraded in the lysosome. Downregulation of CXCR4 could also occur through the stimulation of other GPCRs8.The distribution of CXCR4 is quite broad and involves the central nervous system (CNS)9, spleen10, testes11, hematopoietic and non-hematopoietic cells12.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS

Short Description :

A Rabbit Polyclonal Antibody to CXCR4 Chemokine Receptor

Long Description :

Anti-CXCR4 (extracellular) Antibody (#ACR-014) is a highly specific antibody directed against an extracellular epitope of the human CXCR4 chemokine receptor. The antibody can be used for western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize CXCR4 from rat, mouse and human samples.

Negative Control :

BLP-CR014

Positive Control :

Synonyms :

C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CXCR4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage