antibody

Anti-CXCR4 (extracellular)-FITC Antibody

ACR-014-F

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ACR-014-F_15 mcl

Anti-CXCR4 (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

P61073

FC LCI

Human Rat Mouse

Rabbit

BLP-CR014

Mouse - 12/14 amino acid residues identical; rat - 9/14 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4

Chemokines are small molecular weight, soluble secreted proteins that bind and activate their respective G-protein coupled receptor (GPCR), chemokine receptors in order to evoke a cellular response resulting in migration or chemotaxis1.The chemokine system involves more than 40 chemokines and 18 chemokine receptors. The receptors are designated CXCR1-5, CCR1-11, XCR1 and CX3CR1, based on their specific ligand preference2.Chemokine receptors are present on many different cell types. They were initially detected on leukocytes, where they were found to play an important role in the migration of these cells to inflammation sites3.CXCR4 was originally identified as an orphan receptor, and soon gained much attention when it was discovered as a coreceptor for HIV-14. Besides from being involved in HIV-1 infection/progression, CXCR4 is found to be upregulated in many different cancers/tumors and has evolved to become a target for the development of antagonists5. CXCL12 (SDF-1α) is the sole ligand for CXCR4. Following binding of its ligand, CXCR4 undergoes dimerization and activates Gi G-proteins6,7. However downstream activation through CXCR4 could also occur through other G-proteins and non-G-proteins5. The down regulation of the CXCR4 receptor is initiated by phosphorylation of its cytoplasmic tail, which is followed by the binding of arrestin. The receptor is then internalized through endocytosis and degraded in the lysosome. Downregulation of CXCR4 could also occur through the stimulation of other GPCRs8.The distribution of CXCR4 is quite broad and involves the central nervous system (CNS)9, spleen10, testes11, hematopoietic and non-hematopoietic cells12.

FC LCI

Alomone Labs

C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS

A Rabbit Polyclonal Antibody to CXCR4 (extracellular) conjugated to the fluorescent dye FITC

Anti-CXCR4 (extracellular) Antibody (#ACR-014) is a highly specific antibody directed against an extracellular epitope of the human CXCR4 chemokine receptor. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CXCR4 from mouse, rat and human samples. Anti-CXCR4 (extracellular)-FITC Antibody (#ACR-014-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize CXCR4 from mouse, rat and human samples.

RIC-001-F

NA

C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

CXCR4

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ACR-014), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information